C57BL/6JCya-Pdxkem1/Cya
Common Name:
Pdxk-KO
Product ID:
S-KO-16601
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pdxk-KO
Strain ID
KOCMP-216134-Pdxk-B6J-VA
Gene Name
Product ID
S-KO-16601
Gene Alias
2310036D04Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pdxkem1/Cya mice (Catalog S-KO-16601) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041616
NCBI RefSeq
NM_172134
Target Region
Exon 2~4
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
Pdxk, or pyridoxal kinase, is a key enzyme in the vitamin B6 salvage pathway, converting inactive B6 vitamers to the active cofactor pyridoxal 5'-phosphate (PLP). PLP is implicated in numerous crucial metabolic reactions, underlining the importance of Pdxk in maintaining normal cellular functions [5,7].
Mutations in PDXK lead to autosomal recessive axonal peripheral polyneuropathy with optic atrophy due to reduced PDXK enzymatic activity and low PLP levels, which can be rescued with PLP supplementation [2,5]. In hepatocellular carcinoma (HCC), upregulated PDXK promotes proliferation and metastasis, and PDXK deficiency facilitates cuproptosis, suggesting it could be a diagnostic and therapeutic target [1]. In serous ovarian cancer, PDXK promotes cell proliferation and is associated with poor prognosis [4]. The natural product P57 induces hypothermia by inhibiting PDXK kinase activity, causing PL accumulation in the hypothalamus [3]. In the context of spinal cord injury, overexpression of PDXK in the motor cortex enhances neuronal growth and locomotor function, while miR-339 targeting PDXK facilitates neurological recovery [6].
In summary, Pdxk is essential for the production of active vitamin B6, which is involved in multiple metabolic reactions. Research on Pdxk, especially through studies of its mutations and functional changes, has revealed its significance in various disease conditions, including neuropathies, cancers, and thermoregulation. These findings provide potential directions for diagnosis, treatment, and further exploration of the underlying molecular mechanisms in these diseases.
References:
1. Chen, Yuqiao, Tang, Lu, Huang, Wentao, Zhang, Gewen, Yao, Lei. 2023. Identification of a prognostic cuproptosis-related signature in hepatocellular carcinoma. In Biology direct, 18, 4. doi:10.1186/s13062-023-00358-w. https://pubmed.ncbi.nlm.nih.gov/36750831/
2. Chelban, Viorica, Wilson, Matthew P, Warman Chardon, Jodi, Clayton, Peter T, Houlden, Henry. 2019. PDXK mutations cause polyneuropathy responsive to pyridoxal 5'-phosphate supplementation. In Annals of neurology, 86, 225-240. doi:10.1002/ana.25524. https://pubmed.ncbi.nlm.nih.gov/31187503/
3. Wang, Ruina, Xiao, Lei, Pan, Jianbo, Yu, Biao, Dang, Yongjun. 2023. Natural product P57 induces hypothermia through targeting pyridoxal kinase. In Nature communications, 14, 5984. doi:10.1038/s41467-023-41435-y. https://pubmed.ncbi.nlm.nih.gov/37752106/
4. Tan, Wei, Liu, Bin, Ling, Huhua. . [Pyridoxal kinase (PDXK) promotes the proliferation of serous ovarian cancer cells and is associated with poor prognosis]. In Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 36, 542-548. doi:. https://pubmed.ncbi.nlm.nih.gov/32696745/
5. Keller, Natalie, Mendoza-Ferreira, Natalia, Maroofian, Reza, Wirth, Brunhilde, Karakaya, Mert. 2020. Hereditary polyneuropathy with optic atrophy due to PDXK variant leading to impaired Vitamin B6 metabolism. In Neuromuscular disorders : NMD, 30, 583-589. doi:10.1016/j.nmd.2020.04.004. https://pubmed.ncbi.nlm.nih.gov/32522499/
6. Xiong, Liu-Lin, Qin, Yan-Xia, Xiao, Qiu-Xia, Bai, Xue, Wang, Ting-Hua. 2020. MicroRNA339 Targeting PDXK Improves Motor Dysfunction and Promotes Neurite Growth in the Remote Cortex Subjected to Spinal Cord Transection. In Frontiers in cell and developmental biology, 8, 577. doi:10.3389/fcell.2020.00577. https://pubmed.ncbi.nlm.nih.gov/32793586/
7. Mascolo, Elisa, Barile, Anna, Mecarelli, Lorenzo Stufera, Contestabile, Roberto, Vernì, Fiammetta. 2019. The expression of four pyridoxal kinase (PDXK) human variants in Drosophila impacts on genome integrity. In Scientific reports, 9, 14188. doi:10.1038/s41598-019-50673-4. https://pubmed.ncbi.nlm.nih.gov/31578392/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen