C57BL/6JCya-Cyp2c23em1/Cya
Common Name:
Cyp2c23-KO
Product ID:
S-KO-16928
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cyp2c23-KO
Strain ID
KOCMP-226143-Cyp2c23-B6J-VB
Gene Name
Product ID
S-KO-16928
Gene Alias
Cyp2c44; b2b386Clo
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp2c23em1/Cya mice (Catalog S-KO-16928) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026211
NCBI RefSeq
NM_001001446
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Cyp2c23, a member of the cytochrome P450 2C subfamily, is constitutively expressed in rat liver and kidney. It functions as an arachidonic acid epoxygenase, efficiently producing epoxyeicosatrienoic acids (EETs) such as 8,9-EET, 11,12-EET, and 14,15-EET in the kidney [2]. These EETs are involved in regulating renal vascular tone, salt excretion, and have anti-inflammatory properties. The gene is also important in the context of various physiological and pathological processes in rats [3,4,5].
In rats, low Na intake downregulates the activity and expression of Cyp2c23 in the thick ascending limb and collecting duct of the kidney, attenuating the inhibitory effect of arachidonic acid on epithelial Na channels and Na transport [1]. A peroxisome proliferator-activated receptor-α activator, fenofibrate, can induce renal Cyp2c23 protein and AA-epoxygenase activity, protecting against angiotensin II-induced renal injury [3]. In a rodent model of septic shock, increased Cyp2c23 expression is associated with the protective effect of 5,14-HEDGE against hypotension, tachycardia, inflammation, and mortality [4]. Also, in salt-sensitive hypertension, tumor necrosis factor alpha blockade increases renal Cyp2c23 expression and slows the progression of renal damage [5].
In conclusion, Cyp2c23 plays crucial roles in renal function, blood pressure regulation, and inflammation-related processes in rats. These functions are revealed through in-vivo studies in rats, which are important research models. Understanding Cyp2c23's functions provides insights into the mechanisms underlying diseases related to renal function and blood pressure regulation, potentially guiding the development of new therapeutic strategies.
References:
1. Sun, Peng, Lin, Dao-Hong, Wang, Tong, Nasjletti, Alberto, Wang, Wen-Hui. 2006. Low Na intake suppresses expression of CYP2C23 and arachidonic acid-induced inhibition of ENaC. In American journal of physiology. Renal physiology, 291, F1192-200. doi:. https://pubmed.ncbi.nlm.nih.gov/16849695/
2. Imaoka, S, Wedlund, P J, Ogawa, H, Gonzalez, F J, Kim, H Y. . Identification of CYP2C23 expressed in rat kidney as an arachidonic acid epoxygenase. In The Journal of pharmacology and experimental therapeutics, 267, 1012-6. doi:. https://pubmed.ncbi.nlm.nih.gov/8246128/
3. Muller, Dominik N, Theuer, Juergen, Shagdarsuren, Erdenechimeg, Luft, Friedrich C, Schunck, Wolf-Hagen. . A peroxisome proliferator-activated receptor-alpha activator induces renal CYP2C23 activity and protects from angiotensin II-induced renal injury. In The American journal of pathology, 164, 521-32. doi:. https://pubmed.ncbi.nlm.nih.gov/14742258/
4. Tunctan, Bahar, Korkmaz, Belma, Sari, Ayse Nihal, Falck, John R, Malik, Kafait U. 2013. 5,14-HEDGE, a 20-HETE mimetic, reverses hypotension and improves survival in a rodent model of septic shock: contribution of soluble epoxide hydrolase, CYP2C23, MEK1/ERK1/2/IKKβ/IκB-α/NF-κB pathway, and proinflammatory cytokine formation. In Prostaglandins & other lipid mediators, 102-103, 31-41. doi:10.1016/j.prostaglandins.2013.01.005. https://pubmed.ncbi.nlm.nih.gov/23454652/
5. Elmarakby, Ahmed A, Quigley, Jeffrey E, Pollock, David M, Imig, John D. 2006. Tumor necrosis factor alpha blockade increases renal Cyp2c23 expression and slows the progression of renal damage in salt-sensitive hypertension. In Hypertension (Dallas, Tex. : 1979), 47, 557-62. doi:. https://pubmed.ncbi.nlm.nih.gov/16415373/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen