C57BL/6JCya-Dbiem1/Cya
Common Name
Dbi-KO
Product ID
S-KO-17032
Backgroud
C57BL/6JCya
Strain ID
KOCMP-13167-Dbi-B6J-VA
When using this mouse strain in a publication, please cite “Dbi-KO Mouse (Catalog S-KO-17032) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Dbi-KO
Strain ID
KOCMP-13167-Dbi-B6J-VA
Gene Name
Product ID
S-KO-17032
Gene Alias
ACBD1, Acbp, EP, endozepine
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000151708
NCBI RefSeq
NM_001037999
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Overview of Gene Research
Dbi, also known as diazepam binding inhibitor (DBI) or acyl-CoA binding protein (ACBP), is a phylogenetically conserved paracrine inhibitor of macroautophagy/autophagy, acting as a pro-aging molecule [1]. It can bind to the GABAA receptor and is involved in regulating lipid metabolism. Genetic loss-of-function models have been crucial in understanding its physiological roles in vivo [2].
In KO mouse models, knockout of ACB1 (yeast equivalent of human Dbi) induces autophagy and prolongs lifespan in an autophagy-dependent manner [1]. Antibody-mediated neutralization of Dbi in mice reduces signs of anthracycline-accelerated cardiac aging, such as the upregulation of the senescence marker CDKN2A/p16 and functional decline of the heart [1]. Also, in adipose tissue-specific Acbp/Dbi knockout mice, high-fat diet-induced weight gain is prevented [3].
In conclusion, Dbi plays essential roles in regulating autophagy, lipid metabolism, and body mass homeostasis. Its function has been revealed through gene knockout models, which have also demonstrated its significance in age-associated cardiovascular diseases and obesity-related conditions.
References:
1. Montégut, Léa, Joseph, Adrien, Chen, Hui, Madeo, Frank, Kroemer, Guido. 2022. DBI/ACBP is a targetable autophagy checkpoint involved in aging and cardiovascular disease. In Autophagy, 19, 2166-2169. doi:10.1080/15548627.2022.2160565. https://pubmed.ncbi.nlm.nih.gov/36579946/
2. Alquier, Thierry, Christian-Hinman, Catherine A, Alfonso, Julieta, Færgeman, Nils J. 2021. From benzodiazepines to fatty acids and beyond: revisiting the role of ACBP/DBI. In Trends in endocrinology and metabolism: TEM, 32, 890-903. doi:10.1016/j.tem.2021.08.009. https://pubmed.ncbi.nlm.nih.gov/34565656/
3. Joseph, Adrien, Chen, Hui, Anagnostopoulos, Gerasimos, Martins, Isabelle, Kroemer, Guido. 2021. Effects of acyl-coenzyme A binding protein (ACBP)/diazepam-binding inhibitor (DBI) on body mass index. In Cell death & disease, 12, 599. doi:10.1038/s41419-021-03864-9. https://pubmed.ncbi.nlm.nih.gov/34108446/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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