C57BL/6JCya-Zdhhc12em1/Cya
Common Name:
Zdhhc12-KO
Product ID:
S-KO-17419
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zdhhc12-KO
Strain ID
KOCMP-66220-Zdhhc12-B6J-VA
Gene Name
Product ID
S-KO-17419
Gene Alias
1190004A01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zdhhc12em1/Cya mice (Catalog S-KO-17419) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000081838
NCBI RefSeq
NM_025428
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Zdhhc12, a zinc finger DHHC-type palmitoyltransferase, is involved in the S-palmitoylation of various proteins. S-palmitoylation is a post-translational modification where a palmitate group is attached to cysteine residues, which can regulate protein localization, stability, and function. This process participates in multiple biological pathways, including those related to inflammation, tumorigenesis, and immune responses [1,2,3,4,5,6,7,8].
In mice, Zdhhc12 deficiency enhances inflammatory symptoms and lethality following alum-induced peritonitis and LPS-induced endotoxic shock, suggesting its role in controlling inflammation by regulating NLRP3 inflammasome activation through promoting NLRP3 degradation via chaperone-mediated autophagy [2,4]. In ovarian cancer, knockdown of ZDHHC12 inhibits CLDN3 accurate membrane localization, protein stability, and ovarian cancer cells tumorigenesis, indicating its role in tumor progression [1]. Also, inhibition of ZDHHC12 in ovarian cancer cells can disrupt mitochondrial function and ROS homeostasis, sensitizing cells to cisplatin [3].
In conclusion, Zdhhc12 is crucial for regulating protein function through S-palmitoylation in processes related to inflammation and cancer. Studies using Zdhhc12-deficient mouse models have revealed its significance in controlling inflammation and its potential as a target in ovarian cancer treatment, providing insights into disease mechanisms and possible therapeutic strategies.
References:
1. Yuan, Meng, Chen, Xiaobing, Sun, Yitang, Cao, Ji, He, Qiaojun. 2020. ZDHHC12-mediated claudin-3 S-palmitoylation determines ovarian cancer progression. In Acta pharmaceutica Sinica. B, 10, 1426-1439. doi:10.1016/j.apsb.2020.03.008. https://pubmed.ncbi.nlm.nih.gov/32963941/
2. Wang, Liqiu, Cai, Jing, Zhao, Xin, Jin, Shouheng, Cui, Jun. 2022. Palmitoylation prevents sustained inflammation by limiting NLRP3 inflammasome activation through chaperone-mediated autophagy. In Molecular cell, 83, 281-297.e10. doi:10.1016/j.molcel.2022.12.002. https://pubmed.ncbi.nlm.nih.gov/36586411/
3. Zhang, Xining, Liao, Xingming, Wang, Min, Cheng, Hailing, Liu, Pixu. 2024. Inhibition of palmitoyltransferase ZDHHC12 sensitizes ovarian cancer cells to cisplatin through ROS-mediated mechanisms. In Cancer science, 115, 1170-1183. doi:10.1111/cas.16085. https://pubmed.ncbi.nlm.nih.gov/38287874/
4. Wang, Liqiu, Cui, Jun. 2023. Palmitoylation promotes chaperone-mediated autophagic degradation of NLRP3 to modulate inflammation. In Autophagy, 19, 2821-2823. doi:10.1080/15548627.2023.2187957. https://pubmed.ncbi.nlm.nih.gov/36927399/
5. Wang, Liqiu, Li, Mengqiu, Lian, Guangyu, Wu, Yaoxing, Cui, Jun. 2024. Palmitoylation acts as a checkpoint for MAVS aggregation to promote antiviral innate immune responses. In The Journal of clinical investigation, 134, . doi:10.1172/JCI177924. https://pubmed.ncbi.nlm.nih.gov/39621307/
6. Yang, Shuai, Li, Mengqiu, Lian, Guangyu, Cui, Jun, Wang, Liqiu. 2024. ABHD8 antagonizes inflammation by facilitating chaperone-mediated autophagy-mediated degradation of NLRP3. In Autophagy, 21, 338-351. doi:10.1080/15548627.2024.2395158. https://pubmed.ncbi.nlm.nih.gov/39225180/
7. Jiang, Lu, Wang, Zirui, Xu, Ting, Zhang, Leiliang. 2024. When pyro(ptosis) meets palm(itoylation). In Cytokine & growth factor reviews, 77, 30-38. doi:10.1016/j.cytogfr.2024.03.001. https://pubmed.ncbi.nlm.nih.gov/38472042/
8. Zhu, Xue-Xue, Meng, Xin-Yu, Zhang, Ao-Yuan, Qiu, Li-Ying, Sun, Hai-Jian. 2024. Vaccarin alleviates septic cardiomyopathy by potentiating NLRP3 palmitoylation and inactivation. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 131, 155771. doi:10.1016/j.phymed.2024.155771. https://pubmed.ncbi.nlm.nih.gov/38851101/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen