C57BL/6JCya-Asap1em1/Cya
Common Name:
Asap1-KO
Product ID:
S-KO-17522
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Asap1-KO
Strain ID
KOCMP-13196-Asap1-B6J-VB
Gene Name
Product ID
S-KO-17522
Gene Alias
DEF-1; Ddef1; PAP; mKIAA1249; s19
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asap1em1/Cya mice (Catalog S-KO-17522) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000177374
NCBI RefSeq
NM_010026
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
ASAP1, an ADP ribosylation factor (ARF) GTPase activation protein, has multiple functions including regulating cytoskeletal dynamics, small GTP - binding protein receptor recycling, and intracellular vesicle trafficking [5]. It is involved in pathways such as the IQGAP1/CDC42 pathway, which is related to tumor progression and chemotherapy resistance [1]. It also has a role in actin stress fiber organization via its N - BAR domain, contributing to cell migration, spreading, and focal adhesion dynamics [8].
In gastric cancer, ASAP1 upregulation promotes cell proliferation, migration, invasion, and chemotherapy resistance by inhibiting IQGAP1 ubiquitination and activating the CDC42 - EGFR - MAPK pathway [1]. In glioblastoma, EIF4A3 - induced circular RNA ASAP1 promotes tumorigenesis and temozolomide resistance via the NRAS/MEK1/ERK1 - 2 signaling pathway [2]. In invasive breast cancer, low ASAP1 expression is associated with worse recurrence - free survival, especially in ER - positive cases [3]. In hepatocellular carcinoma, high ASAP1 expression is linked to aggressive clinicopathological features and poor outcomes [4]. In zebrafish, Asap1 affects susceptibility to Mycobacterium by regulating macrophage migration [5]. In NSCLC, lncRNA ASAP1 - IT1 enhances cancer cell stemness via the miR - 509 - 3p/YAP1 axis [6]. In the MMTV - PyMT model of luminal breast cancer, loss of ASAP1 activates AKT, accelerates tumorigenesis, and promotes metastasis [7]. In extrahepatic cholangiocarcinoma, ASAP1 promotes tumor development by regulating the Wnt/β - catenin pathway [9]. In gastric cancer, high expression of ASAP1 is associated with depth of invasion, lymph node metastasis, pathological stage, and poor survival, and is positively associated with FAK expression [10].
In conclusion, ASAP1 plays crucial roles in multiple biological processes, especially in tumor - related events such as tumor progression, metastasis, and chemotherapy resistance across various cancer types. Studies using different models, including zebrafish and mouse models, have provided insights into its functions in disease conditions, highlighting its potential as a biomarker and therapeutic target.
References:
1. Xie, Wangkai, Han, Zheng, Zuo, Ziyi, Xue, Xiangyang, Lin, Kezhi. 2023. ASAP1 activates the IQGAP1/CDC42 pathway to promote tumor progression and chemotherapy resistance in gastric cancer. In Cell death & disease, 14, 124. doi:10.1038/s41419-023-05648-9. https://pubmed.ncbi.nlm.nih.gov/36792578/
2. Wei, Yutian, Lu, Chenfei, Zhou, Peng, Shi, ZhuMei, You, Yongping. . EIF4A3-induced circular RNA ASAP1 promotes tumorigenesis and temozolomide resistance of glioblastoma via NRAS/MEK1/ERK1-2 signaling. In Neuro-oncology, 23, 611-624. doi:10.1093/neuonc/noaa214. https://pubmed.ncbi.nlm.nih.gov/32926734/
3. Park, Hosub, Son, Hwangkyu, Cha, Hyebin, Chung, Min Sung, Paik, Seungsam. 2023. ASAP1 Expression in Invasive Breast Cancer and Its Prognostic Role. In International journal of molecular sciences, 24, . doi:10.3390/ijms241814355. https://pubmed.ncbi.nlm.nih.gov/37762658/
4. Bang, Seongsik, Jee, Seungyun, Son, Hwangkyu, Kim, Hyunsung, Paik, Seungsam. 2022. Clinicopathological Implications of ASAP1 Expression in Hepatocellular Carcinoma. In Pathology oncology research : POR, 28, 1610635. doi:10.3389/pore.2022.1610635. https://pubmed.ncbi.nlm.nih.gov/36110251/
5. Cui, Jia, Chen, Guangxin, Wen, Da, Zhao, Zhonghua, Wu, Changxin. 2020. Asap1 Affects the Susceptibility of Zebrafish to Mycobacterium by Regulating Macrophage Migration. In Frontiers in cellular and infection microbiology, 10, 519503. doi:10.3389/fcimb.2020.519503. https://pubmed.ncbi.nlm.nih.gov/33194781/
6. Liu, Yantao, Yang, Yuping, Zhang, Lingli, Chen, Kangwu, Zhao, Wei. 2021. LncRNA ASAP1-IT1 enhances cancer cell stemness via regulating miR-509-3p/YAP1 axis in NSCLC. In Cancer cell international, 21, 572. doi:10.1186/s12935-021-02270-7. https://pubmed.ncbi.nlm.nih.gov/34715859/
7. Schreiber, Caroline, Gruber, Annette, Roßwag, Sven, Garvalov, Boyan K, Sleeman, Jonathan P. 2022. Loss of ASAP1 in the MMTV-PyMT model of luminal breast cancer activates AKT, accelerates tumorigenesis, and promotes metastasis. In Cancer letters, 533, 215600. doi:10.1016/j.canlet.2022.215600. https://pubmed.ncbi.nlm.nih.gov/35181478/
8. Gasilina, Anjelika, Vitali, Teresa, Luo, Ruibai, Jian, Xiaoying, Randazzo, Paul A. 2019. The ArfGAP ASAP1 Controls Actin Stress Fiber Organization via Its N-BAR Domain. In iScience, 22, 166-180. doi:10.1016/j.isci.2019.11.015. https://pubmed.ncbi.nlm.nih.gov/31785555/
9. He, Jiaqi, Liu, Han, Cai, Jianhua, Wang, Jiwen, Liu, Houbao. 2024. ASAP1 promotes extrahepatic cholangiocarcinoma progression by regulating the Wnt/β-catenin pathway in vitro and in vivo. In American journal of cancer research, 14, 5178-5192. doi:10.62347/RKQX3504. https://pubmed.ncbi.nlm.nih.gov/39659924/
10. Luo, Qiong, Zhang, Suyun, Zhang, Donghuan, Chen, Xiangqi, Yang, Sheng. 2020. Expression of ASAP1 and FAK in gastric cancer and its clinicopathological significance. In Oncology letters, 20, 974-980. doi:10.3892/ol.2020.11612. https://pubmed.ncbi.nlm.nih.gov/32566028/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen