Trim27, also known as RFP, is a RING-mediated E3 ubiquitin ligase. It can mark proteins like PTEN, IκBα, and p53 for degradation, regulating multiple signaling pathways related to cell proliferation, differentiation, and apoptosis [2]. It is involved in diverse biological processes, with its functions studied through genetic models such as gene knockout mouse models.

In knockout mouse models, Trim27 deficiency leads to IBS-like symptoms including increased visceral hyperalgesia and abnormal stool features, as TRIM27 stabilizes β-catenin to activate Wnt/β-catenin signaling for intestinal stem cell self-renewal, indicating its crucial role in gut homeostasis [1]. Also, Trim27 knockout mice show increased basal autophagy levels, and Trim27 cooperates with STK38L to inhibit ULK1-mediated autophagy, influencing tumorigenesis and metastasis [3].

In conclusion, Trim27 plays essential roles in maintaining gut homeostasis and regulating autophagy, which are associated with diseases like IBS and cancer. The study of Trim27 using knockout mouse models has provided insights into the mechanisms of these diseases, potentially guiding the development of new treatment strategies.