C57BL/6JCya-Trim27em1/Cya
Common Name
Trim27-KO
Product ID
S-KO-17674
Backgroud
C57BL/6JCya
Strain ID
KOCMP-19720-Trim27-B6J-VB
When using this mouse strain in a publication, please cite “Trim27-KO Mouse (Catalog S-KO-17674) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Trim27-KO
Strain ID
KOCMP-19720-Trim27-B6J-VB
Gene Name
Product ID
S-KO-17674
Gene Alias
Gm19403, Rfp
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 13
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000021761
NCBI RefSeq
NM_009054
Target Region
Exon 5~6
Size of Effective Region
~1.6 kb
Overview of Gene Research
Trim27, also known as RFP, is a RING-mediated E3 ubiquitin ligase. It can mark proteins like PTEN, IκBα, and p53 for degradation, regulating multiple signaling pathways related to cell proliferation, differentiation, and apoptosis [2]. It is involved in diverse biological processes, with its functions studied through genetic models such as gene knockout mouse models.
In knockout mouse models, Trim27 deficiency leads to IBS-like symptoms including increased visceral hyperalgesia and abnormal stool features, as TRIM27 stabilizes β-catenin to activate Wnt/β-catenin signaling for intestinal stem cell self-renewal, indicating its crucial role in gut homeostasis [1]. Also, Trim27 knockout mice show increased basal autophagy levels, and Trim27 cooperates with STK38L to inhibit ULK1-mediated autophagy, influencing tumorigenesis and metastasis [3].
In conclusion, Trim27 plays essential roles in maintaining gut homeostasis and regulating autophagy, which are associated with diseases like IBS and cancer. The study of Trim27 using knockout mouse models has provided insights into the mechanisms of these diseases, potentially guiding the development of new treatment strategies.
References：
1. Wang, Jing, Zhao, Dongdong, Lei, Zehui, Zhang, Lingqiang, Liu, Cui Hua. 2023. TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal. In Cellular & molecular immunology, 20, 158-174. doi:10.1038/s41423-022-00963-1. https://pubmed.ncbi.nlm.nih.gov/36596873/
2. Yu, Chengpeng, Rao, Dean, Wang, Tiantian, Zhang, Lei, Huang, Wenjie. 2022. Emerging roles of TRIM27 in cancer and other human diseases. In Frontiers in cell and developmental biology, 10, 1004429. doi:10.3389/fcell.2022.1004429. https://pubmed.ncbi.nlm.nih.gov/36200036/
3. Yang, Yi, Zhu, Yifu, Zhou, Shuai, Wu, Mian, Chen, Song. 2022. TRIM27 cooperates with STK38L to inhibit ULK1-mediated autophagy and promote tumorigenesis. In The EMBO journal, 41, e109777. doi:10.15252/embj.2021109777. https://pubmed.ncbi.nlm.nih.gov/35670107/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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