C57BL/6JCya-Pi16em1/Cya
Common Name:
Pi16-KO
Product ID:
S-KO-17969
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pi16-KO
Strain ID
KOCMP-74116-Pi16-B6J-VB
Gene Name
Product ID
S-KO-17969
Gene Alias
1200009H11Rik; Cripi; PI-16
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pi16em1/Cya mice (Catalog S-KO-17969) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114701
NCBI RefSeq
NM_023734.3
Target Region
Exon 3~4
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Pi16, also known as peptidase inhibitor 16, is a gene with diverse functions. Fibroblast-derived Pi16 is involved in immune-modulation, pain regulation, and cancer-related processes [1,2,3,4,5,6,7]. In pain regulation, it may act through influencing immune cell-fibroblast crosstalk; in cancer, it can have either tumor-suppressive or tumor-promoting roles depending on the context [1,3,4,5].
In gene knockout (KO) mouse models, Pi16-/-mice are protected against sustained inflammatory pain, likely due to reduced macrophage density and a bias towards anti-inflammatory CD206hi macrophages in the pain neuroaxis [1]. In neuropathic pain, Pi16-/-mice show reduced endothelial barrier permeability, lower leukocyte infiltration, and are protected against neuropathic pain, suggesting Pi16 promotes pain by mediating fibroblast-endothelial barrier cross-talk [7]. Conditional knockout (CKO) of Pi16 in regulatory T cells (Tregs) enhances their differentiation and suppressive functions, protecting mice from autoimmune arthritis and dextran sulfate sodium-induced colitis [8].
In conclusion, Pi16 plays crucial roles in pain, immune regulation, and cancer. KO and CKO mouse models have revealed its functions in inflammatory and neuropathic pain, as well as autoimmune diseases, providing insights into potential therapeutic targets for chronic pain, autoimmune, and inflammatory diseases.
References：
1. Garrity, Rachelle, Arora, Neha, Haque, Md Areeful, Heijnen, Cobi J, Shepherd, Andrew J. 2023. Fibroblast-derived PI16 sustains inflammatory pain via regulation of CD206+ myeloid cells. In Brain, behavior, and immunity, 112, 220-234. doi:10.1016/j.bbi.2023.06.011. https://pubmed.ncbi.nlm.nih.gov/37315702/
2. De Martin, Angelina, Stanossek, Yves, Lütge, Mechthild, Pikor, Natalia B, Ludewig, Burkhard. 2023. PI16+ reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches. In Nature immunology, 24, 1138-1148. doi:10.1038/s41590-023-01502-4. https://pubmed.ncbi.nlm.nih.gov/37202490/
3. Gao, Yang, Li, Jianan, Cheng, Wenfeng, Hou, Wenhong, Zhang, Zemin. 2024. Cross-tissue human fibroblast atlas reveals myofibroblast subtypes with distinct roles in immune modulation. In Cancer cell, 42, 1764-1783.e10. doi:10.1016/j.ccell.2024.08.020. https://pubmed.ncbi.nlm.nih.gov/39303725/
4. Kuang, Xiangqin, Zhang, Zhuojun, Li, Difeng, Yang, Jianan, Jiang, Lili. 2023. Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO. In Cellular & molecular biology letters, 28, 62. doi:10.1186/s11658-023-00465-6. https://pubmed.ncbi.nlm.nih.gov/37525118/
5. Wang, Pusen, Jiang, Zhongyi, Liu, Xueni, Li, Hao, Zhong, Lin. 2020. PI16 attenuates response to sorafenib and represents a predictive biomarker in hepatocellular carcinoma. In Cancer medicine, 9, 6972-6983. doi:10.1002/cam4.3331. https://pubmed.ncbi.nlm.nih.gov/32779397/
6. McCartney, Erika E, Chung, Yein, Buechler, Matthew B. 2024. Life of Pi: Exploring functions of Pi16+ fibroblasts. In F1000Research, 13, 126. doi:. https://pubmed.ncbi.nlm.nih.gov/38919948/
7. Singhmar, Pooja, Trinh, Ronnie The Phong, Ma, Jiacheng, Heijnen, Cobi J, Kavelaars, Annemieke. 2020. The fibroblast-derived protein PI16 controls neuropathic pain. In Proceedings of the National Academy of Sciences of the United States of America, 117, 5463-5471. doi:10.1073/pnas.1913444117. https://pubmed.ncbi.nlm.nih.gov/32079726/
8. Sun, Yuankai, Lin, Shiyu, Wang, Hui, Wang, Fang, Tan, Wenfeng. 2024. Regulatory role of PI16 in autoimmune arthritis and intestinal inflammation: implications for Treg cell differentiation and function. In Journal of translational medicine, 22, 327. doi:10.1186/s12967-024-05082-1. https://pubmed.ncbi.nlm.nih.gov/38566233/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen