C57BL/6JCya-Rrbp1em1/Cya
Common Name:
Rrbp1-KO
Product ID:
S-KO-17984
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Rrbp1-KO
Strain ID
KOCMP-81910-Rrbp1-B6J-VB
Gene Name
Product ID
S-KO-17984
Gene Alias
1700087N07Rik; 5730465C04Rik; ES/130; mKIAA1398; mRRp0; mRRp1.8; mRRp10; mRRp15a; mRRp15b; mRRp16.8; mRRp2; mRRp41; mRRp47; mRRp5.4; p180
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rrbp1em1/Cya mice (Catalog S-KO-17984) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000016072
NCBI RefSeq
NM_024281
Target Region
Exon 2
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Rrbp1, also known as P180, is a ribosome-binding transmembrane protein of the endoplasmic reticulum. It plays crucial roles in multiple biological processes. It is involved in regulating local translation in axons, with axonal endoplasmic reticulum tubules controlling local translation via Rrbp1-mediated ribosome interactions, which is important for neuron development [1]. Additionally, it participates in the mitophagy pathway, as mitochondrial protein import stress can trigger LC3 lipidation through NLRX1 and Rrbp1 [2,8].
In disease-related research, Rrbp1 has been implicated in various cancers. In upper tract urothelial carcinoma, gene body hypomethylation of Rrbp1 is associated with high expression, tumor malignancy, and chemoresistance [3]. In oral squamous cell carcinoma, it drives cisplatin-resistance by regulating the Hippo pathway [4]. In bladder, prostate, and epithelial ovarian cancers, high Rrbp1 expression is related to tumor stage, metastasis, and poor prognosis [5,9,10]. In bone metastatic cancer cells, depletion of Rrbp1 enhances the osteoblastic phenotype expression [6]. In prostate cancer, METTL3 stabilizes Rrbp1 mRNA in an m6A-dependent manner, and inhibition of METTL3 can suppress cancer cell proliferation [7].
In conclusion, Rrbp1 is essential for normal neuron development and is involved in mitophagy. In the context of cancer, Rrbp1 plays significant roles in tumor malignancy, metastasis, and chemoresistance across multiple cancer types. The study of Rrbp1 using gene-knockout or conditional-knockout models, though not explicitly detailed in all references, would further clarify its functions in these biological processes and disease conditions, providing potential therapeutic targets for related diseases.
References:
1. Koppers, Max, Özkan, Nazmiye, Nguyen, Ha H, Hoogenraad, Casper C, Farías, Ginny G. 2024. Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions. In Developmental cell, 59, 2053-2068.e9. doi:10.1016/j.devcel.2024.05.005. https://pubmed.ncbi.nlm.nih.gov/38815583/
2. Killackey, Samuel A, Bi, Yuntian, Soares, Fraser, Arnoult, Damien, Girardin, Stephen E. 2022. Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1. In Molecular cell, 82, 2815-2831.e5. doi:10.1016/j.molcel.2022.06.004. https://pubmed.ncbi.nlm.nih.gov/35752171/
3. Luo, Hao-Lun, Liu, Hui-Ying, Chang, Yin-Lun, Huang, Chun-Chieh, Peng, Jei-Ming. 2021. Hypomethylated RRBP1 Potentiates Tumor Malignancy and Chemoresistance in Upper Tract Urothelial Carcinoma. In International journal of molecular sciences, 22, . doi:10.3390/ijms22168761. https://pubmed.ncbi.nlm.nih.gov/34445467/
4. Shriwas, Omprakash, Arya, Rakesh, Mohanty, Sibasish, Nanda, Ranjan K, Dash, Rupesh. 2021. RRBP1 rewires cisplatin resistance in oral squamous cell carcinoma by regulating Hippo pathway. In British journal of cancer, 124, 2004-2016. doi:10.1038/s41416-021-01336-7. https://pubmed.ncbi.nlm.nih.gov/33762722/
5. Wang, Mingshuai, Liu, Sai, Zhou, Bolin, Ping, Hao, Xing, Nianzeng. 2020. RRBP1 is highly expressed in bladder cancer and is associated with migration and invasion. In Oncology letters, 20, 203. doi:10.3892/ol.2020.12066. https://pubmed.ncbi.nlm.nih.gov/32963609/
6. Chen, Rui, Wang, Yue, Xu, Yang, Xia, Chun, Zhang, Bing. 2022. RRBP1 depletion of bone metastatic cancer cells contributes to enhanced expression of the osteoblastic phenotype. In Frontiers in oncology, 12, 1005152. doi:10.3389/fonc.2022.1005152. https://pubmed.ncbi.nlm.nih.gov/36568157/
7. Feng, Yuqing, Li, Zenghui, Zhu, Jinwei, Shi, Junfeng, Zhang, Dingxiao. 2024. Stabilization of RRBP1 mRNA via an m6A-dependent manner in prostate cancer constitutes a therapeutic vulnerability amenable to small-peptide inhibition of METTL3. In Cellular and molecular life sciences : CMLS, 81, 414. doi:10.1007/s00018-024-05418-6. https://pubmed.ncbi.nlm.nih.gov/39367907/
8. Killackey, Samuel A, Bi, Yuntian, Philpott, Dana J, Arnoult, Damien, Girardin, Stephen E. 2022. Mitochondria-ER cooperation: NLRX1 detects mitochondrial protein import stress and promotes mitophagy through the ER protein RRBP1. In Autophagy, 19, 1601-1603. doi:10.1080/15548627.2022.2129763. https://pubmed.ncbi.nlm.nih.gov/36170592/
9. Li, Tieqiu, Wang, Qianqian, Hong, Xiuqin, Li, Jiahui, Lei, Bin. 2019. RRBP1 is highly expressed in prostate cancer and correlates with prognosis. In Cancer management and research, 11, 3021-3027. doi:10.2147/CMAR.S186632. https://pubmed.ncbi.nlm.nih.gov/31118771/
10. Ma, Jing, Ren, Sujing, Ding, Jing, Ma, Rong, Meng, Fanling. 2019. Expression of RRBP1 in epithelial ovarian cancer and its clinical significance. In Bioscience reports, 39, . doi:10.1042/BSR20190656. https://pubmed.ncbi.nlm.nih.gov/31285390/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen