C57BL/6JCya-Ano10em1/Cya
Common Name:
Ano10-KO
Product ID:
S-KO-17996
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ano10-KO
Strain ID
KOCMP-102566-Ano10-B6J-VB
Gene Name
Product ID
S-KO-17996
Gene Alias
Tmem16k
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ano10em1/Cya mice (Catalog S-KO-17996) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000042546
NCBI RefSeq
NM_133979
Target Region
Exon 7~8
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Ano10, also known as TMEM16K, is a transmembrane protein belonging to the anoctamin family, characterized by functional duality with ion channel and phospholipid scrambling activities. It's involved in multiple physiological processes such as endosomal sorting, spindle assembly, calcium signalling, and is crucial for cell division, migration, apoptosis, and cell signalling [1]. Dysregulation of calcium signalling in Purkinje cells due to ANO10 defects is proposed as the main mechanism leading to spinocerebellar ataxia autosomal recessive type 10 (SCAR10) [1].
In Ano10 knockout epithelial cells, there are defective ion transport, attenuated volume regulation, and deranged Ca2+ signalling. Intestinal epithelial cells from Ano10 null mice are reduced in size and show almost abolished spontaneous and TNFα-induced apoptosis. Similar defects are found in mouse peritoneal Ano10 null macrophages and human THP1 macrophages with reduced ANO10 expression, suggesting that Ano10 mutations cause cellular defects and genetic disorders through deranged local Ca2+ signalling [2].
In conclusion, Ano10 plays essential roles in multiple cellular processes including calcium signalling and cell apoptosis. The study of Ano10 knockout models has revealed its significant contribution to understanding the pathogenesis of SCAR10, highlighting its importance in related neurological disease research.
References:
1. Chrysanthou, Androniki, Ververis, Antonis, Christodoulou, Kyproula. 2022. ANO10 Function in Health and Disease. In Cerebellum (London, England), 22, 447-467. doi:10.1007/s12311-022-01395-3. https://pubmed.ncbi.nlm.nih.gov/35648332/
2. Wanitchakool, Podchanart, Ousingsawat, Jiraporn, Sirianant, Lalida, Schreiber, Rainer, Kunzelmann, Karl. 2016. Cellular defects by deletion of ANO10 are due to deregulated local calcium signaling. In Cellular signalling, 30, 41-49. doi:10.1016/j.cellsig.2016.11.006. https://pubmed.ncbi.nlm.nih.gov/27838374/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen