C57BL/6JCya-Pla2g4cem1/Cya
Common Name:
Pla2g4c-KO
Product ID:
S-KO-18293
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pla2g4c-KO
Strain ID
KOCMP-232889-Pla2g4c-B6J-VB
Gene Name
Product ID
S-KO-18293
Gene Alias
D7Ertd445e
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pla2g4cem1/Cya mice (Catalog S-KO-18293) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108528
NCBI RefSeq
NM_001168504
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Pla2g4c, encoding cytosolic phospholipase A2-gamma (cPLA2-γ), is involved in remodeling cell membranes, producing prostaglandins, and regulating arachidonic acid metabolism pathway [2,3,6]. It is also associated with lipid droplet formation [4]. Genetic studies on its polymorphisms and mutations are valuable for understanding its functions.
A genome-wide association study found that the rs2303744 polymorphism in Pla2g4c, which causes an amino acid substitution, is associated with Multiple System Atrophy. The cPLA2γ-Ile143 isoform encoded by the MSA risk allele has decreased transacylase activity, potentially perturbing membrane phospholipids and α-synuclein biology [1]. In a murine asthma model, the Pla2g4c/COX-2 (Ptgs2)/PGD2 synthase 2 (Hpgds) axis was significantly increased in bronchial smooth muscle tissues of OA-challenged mice, and increased PGD2 might cause airway smooth muscle hyperresponsiveness [2]. Hepatitis C virus (HCV) infection upregulates Pla2g4c, which is important for HCV replication and lipid droplet formation. Both the membrane attachment and enzymatic activity of Pla2g4c are required for lipid droplet biogenesis [3,4]. Polymorphisms in Pla2g4c are associated with schizophrenia risk, illness severity in male patients, and CRC prognosis, especially in stage II disease. The rs251684 variant of Pla2g4c may be associated with autism spectrum disorder in the Northeast Han Chinese population [5,6,7].
In conclusion, Pla2g4c is essential for cell membrane remodeling, prostaglandin production, and lipid droplet formation. Genetic studies, including those on polymorphisms, have revealed its associations with multiple diseases such as Multiple System Atrophy, asthma, HCV-related diseases, schizophrenia, CRC, and autism spectrum disorder. These findings contribute to understanding the biological functions of Pla2g4c and the molecular mechanisms of these diseases.
References:
1. Nakahara, Yasuo, Mitsui, Jun, Date, Hidetoshi, Foroud, Tatiana, Tsuji, Shoji. 2023. Genome-wide association study identifies a new susceptibility locus in PLA2G4C for Multiple System Atrophy. In medRxiv : the preprint server for health sciences, , . doi:10.1101/2023.05.02.23289328. https://pubmed.ncbi.nlm.nih.gov/37425910/
2. Chiba, Yoshihiko, Suto, Wataru, Sakai, Hiroyasu. 2018. Augmented Pla2g4c/Ptgs2/Hpgds axis in bronchial smooth muscle tissues of experimental asthma. In PloS one, 13, e0202623. doi:10.1371/journal.pone.0202623. https://pubmed.ncbi.nlm.nih.gov/30161143/
3. Mishchenko, E L, Makarova, A A, Antropova, E A, Demenkov, P S, Ivanisenko, V A. . Molecular-genetic pathways of hepatitis C virus regulation of the expression of cellular factors PREB and PLA2G4C, which play an important role in virus replication. In Vavilovskii zhurnal genetiki i selektsii, 27, 776-783. doi:10.18699/VJGB-23-90. https://pubmed.ncbi.nlm.nih.gov/38213698/
4. Su, Xi, Liu, Shuhui, Zhang, Xianwen, Pei, Rongjuan, Chen, Xinwen. 2017. Requirement of cytosolic phospholipase A2 gamma in lipid droplet formation. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1862, 692-705. doi:10.1016/j.bbalip.2017.03.007. https://pubmed.ncbi.nlm.nih.gov/28336330/
5. Nadalin, Sergej, Buretić-Tomljanović, Alena. 2017. An association between PLA2G6 and PLA2G4C gene polymorphisms and schizophrenia risk and illness severity in a Croatian population. In Prostaglandins, leukotrienes, and essential fatty acids, 121, 57-59. doi:10.1016/j.plefa.2017.06.003. https://pubmed.ncbi.nlm.nih.gov/28651698/
6. Olsen, Renate S, Andersson, Roland E, Zar, Niklas, Matussek, Andreas, Dimberg, Jan. 2015. Prognostic significance of PLA2G4C gene polymorphism in patients with stage II colorectal cancer. In Acta oncologica (Stockholm, Sweden), 55, 474-9. doi:10.3109/0284186X.2015.1073350. https://pubmed.ncbi.nlm.nih.gov/26364726/
7. Liu, Shicheng, Qiu, Shuang, Lu, Yuping, Liu, Yawen, Jiang, Huiyi. 2016. The rs251684 Variant of PLA2G4C Is Associated with Autism Spectrum Disorder in the Northeast Han Chinese Population. In Genetic testing and molecular biomarkers, 20, 747-752. doi:10.1089/gtmb.2016.0195. https://pubmed.ncbi.nlm.nih.gov/27611910/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen