C57BL/6JCya-Krt5em1/Cya
Common Name:
Krt5-KO
Product ID:
S-KO-18319
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Krt5-KO
Strain ID
KOCMP-110308-Krt5-B6J-VA
Gene Name
Product ID
S-KO-18319
Gene Alias
3300001P10Rik; CK5; K5; Krt2-5; Tfip8
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Krt5em1/Cya mice (Catalog S-KO-18319) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023709
NCBI RefSeq
NM_027011
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Krt5, also known as keratin 5, is a key intermediate filament protein. It is involved in maintaining the structural integrity of epithelial cells and is associated with multiple biological processes. In the context of signaling pathways, it has been linked to the Notch signaling pathway, which is crucial for cell-cell communication, differentiation, and development [4].
In the lung, Krt5-related research has shown significant findings. In idiopathic pulmonary fibrosis (IPF), the extracellular matrix (ECM) modulates Krt5+ basal cell activity. Changes in ECM composition, like over-expression of SPARC in IPF lung fibroblasts, restrict Krt5+ cell migration in vitro, influencing remodelling events in the fibrotic niche [1]. Also, human alveolar type 2 cells can transdifferentiate into metaplastic Krt5+ basal cells in response to fibrotic signalling in the lung mesenchyme, both in vitro and in vivo [2]. Moreover, p63(+)Krt5(+) distal airway stem cells are essential for lung regeneration. Ablation of these cells in vivo prevents lung regeneration after influenza-induced damage, leading to pre-fibrotic lesions and deficient oxygen exchange [3].
In melanoma, knockdown of Krt5 promoted the proliferation, migration, and invasion of A375 cells, indicating its inhibitory role in melanoma metastasis [5].
In the kidney, Krt5+ urothelial cells act as developmental and tissue repair progenitors, differentiating into Upk+ RUCs during normal ontogeny and after congenital urinary tract obstruction (UTO) [6].
In conclusion, Krt5 is essential for maintaining epithelial integrity and is involved in various biological processes such as cell differentiation, tissue regeneration, and response to disease. Studies, especially those using in vivo models like gene-knockout mouse models, have revealed its significance in diseases including pulmonary fibrosis, melanoma, and kidney obstructive nephropathy. Understanding Krt5 provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Hewitt, Richard J, Puttur, Franz, Gaboriau, David C A, Maher, Toby M, Lloyd, Clare M. 2023. Lung extracellular matrix modulates KRT5+ basal cell activity in pulmonary fibrosis. In Nature communications, 14, 6039. doi:10.1038/s41467-023-41621-y. https://pubmed.ncbi.nlm.nih.gov/37758700/
2. Kathiriya, Jaymin J, Wang, Chaoqun, Zhou, Minqi, Chapman, Harold A, Peng, Tien. 2021. Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells. In Nature cell biology, 24, 10-23. doi:10.1038/s41556-021-00809-4. https://pubmed.ncbi.nlm.nih.gov/34969962/
3. Zuo, Wei, Zhang, Ting, Wu, Daniel Zheng'An, Xian, Wa, McKeon, Frank. 2014. p63(+)Krt5(+) distal airway stem cells are essential for lung regeneration. In Nature, 517, 616-20. doi:10.1038/nature13903. https://pubmed.ncbi.nlm.nih.gov/25383540/
4. Jia, Weixue, Zhang, Yuanyuan, Wang, Xue, Mo, Ran, Li, Chengrang. 2023. KRT5 mutation regulate melanin metabolism through notch signalling pathway between keratinocytes and melanocytes. In Experimental dermatology, 32, 752-765. doi:10.1111/exd.14761. https://pubmed.ncbi.nlm.nih.gov/36809573/
5. Xie, Renjian, Li, Bifei, Jia, Lee, Li, Yumei. 2022. Identification of Core Genes and Pathways in Melanoma Metastasis via Bioinformatics Analysis. In International journal of molecular sciences, 23, . doi:10.3390/ijms23020794. https://pubmed.ncbi.nlm.nih.gov/35054979/
6. Jackson, Ashley R, Hoff, Monica L, Li, Birong, McHugh, Kirk M, Becknell, Brian. 2019. Krt5+ urothelial cells are developmental and tissue repair progenitors in the kidney. In American journal of physiology. Renal physiology, 317, F757-F766. doi:10.1152/ajprenal.00171.2019. https://pubmed.ncbi.nlm.nih.gov/31322419/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen