C57BL/6JCya-Palldem1/Cya
Common Name:
Palld-KO
Product ID:
S-KO-18361
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Palld-KO
Strain ID
KOCMP-72333-Palld-B6J-VA
Gene Name
Product ID
S-KO-18361
Gene Alias
2410003B16Rik; 6030492A02
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Palldem1/Cya mice (Catalog S-KO-18361) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000121785
NCBI RefSeq
NM_001293772
Target Region
Exon 12
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Palladin (PALLD) is an actin-associated protein that belongs to the PALLD/myopalladin (MYPN)/myotilin family. It plays essential roles in multiple biological processes, mainly through its involvement in regulating the actin cytoskeleton. It has been associated with pathways related to cell differentiation, phagocytosis, and muscle-related functions, which are crucial for normal physiological activities and tissue development [2,3,4]. Genetic models, such as knockout (KO) and conditional knockout (CKO) mouse models, have been valuable for studying PALLD's functions.
In a megakaryocyte/platelet-specific KO mouse model, PALLD deficiency led to thrombocytopenia due to diminished platelet biogenesis, impaired proplatelet formation, and polyploidization in megakaryocytes. Mechanistically, PALLD binds to STAT3, and its absence attenuates STAT3 Y705 phosphorylation and impedes STAT3 nuclear translocation [1]. In adult cardiomyocyte-specific inducible CKO mice, ablation of PALLD caused progressive cardiac dilation, systolic dysfunction, reduced cardiomyocyte contractility, intercalated disc abnormalities, and fibrosis, indicating its essential role in normal cardiac function [3].
In conclusion, PALLD is crucial for processes like megakaryocyte differentiation and normal cardiac function. Studies using KO and CKO mouse models have revealed its significance in thrombocytopenia and cardiomyopathy, respectively. These findings enhance our understanding of the biological functions of PALLD and provide potential targets for treating related diseases.
References:
1. Li, Guoming, Jiang, Haojie, Wang, Lingbin, Liu, Junling, Xu, Yanyan. 2024. The role of PALLD-STAT3 interaction in megakaryocyte differentiation and thrombocytopenia treatment. In Haematologica, 109, 3693-3704. doi:10.3324/haematol.2024.285242. https://pubmed.ncbi.nlm.nih.gov/38813732/
2. Sun, Hai-Min, Chen, Xin-Lei, Chen, Xin-Jie, Chen, Zhu, Chen, Sai-Juan. 2017. PALLD Regulates Phagocytosis by Enabling Timely Actin Polymerization and Depolymerization. In Journal of immunology (Baltimore, Md. : 1950), 199, 1817-1826. doi:10.4049/jimmunol.1602018. https://pubmed.ncbi.nlm.nih.gov/28739877/
3. Mastrototaro, Giuseppina, Carullo, Pierluigi, Zhang, Jianlin, Boncompagni, Simona, Bang, Marie-Louise. 2023. Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities. In eLife, 12, . doi:10.7554/eLife.78629. https://pubmed.ncbi.nlm.nih.gov/36927816/
4. Li, Long, Cheng, Xiaofang, Chen, Ling, Luo, Wenzhe, Li, Changchun. 2019. Long Noncoding Ribonucleic Acid MSTRG.59589 Promotes Porcine Skeletal Muscle Satellite Cells Differentiation by Enhancing the Function of PALLD. In Frontiers in genetics, 10, 1220. doi:10.3389/fgene.2019.01220. https://pubmed.ncbi.nlm.nih.gov/31850071/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen