C57BL/6JCya-Rbbp6em1/Cya
Common Name:
Rbbp6-KO
Product ID:
S-KO-18451
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rbbp6-KO
Strain ID
KOCMP-19647-Rbbp6-B6J-VB
Gene Name
Product ID
S-KO-18451
Gene Alias
4933422O15Rik; C030034J04Rik; P2P-R; PACT
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbbp6em1/Cya mice (Catalog S-KO-18451) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052135
NCBI RefSeq
NM_011247
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Rbbp6, short for Retinoblastoma binding protein 6, is a cancer-related protein with E3 ubiquitin ligase activity. It is involved in regulating the cell cycle, apoptosis, and pre-mRNA 3' end processing. Rbbp6 has multiple isoforms, with isoform 1 interacting with tumor suppressors p53 and pRB, and regulating p53 through its ubiquitin ligase activity [2].
In glioblastoma, Nuclease technology knockout screens revealed Rbbp6 as essential for glioblastoma stem cell (GSC) maintenance. Targeting Rbbp6 inhibited GSC proliferation and tumor initiation. Mechanistically, it mediates K63-linked ubiquitination of CPSF3, regulating alternative polyadenylation, and ultimately maintaining high MYC expression in GSCs [1].
In diabetic kidney disease, Rbbp6 promotes the degradation of ERRα through K48-linked polyubiquitination, contributing to mitochondrial injury in proximal renal tubular cells. Conditional ERRα overexpression or RbbP6 inhibition reduced mitochondrial damage in diabetic mice [3].
In summary, Rbbp6 plays crucial roles in multiple biological processes and disease conditions. Gene knockout-based research, such as in glioblastoma and diabetic kidney disease models, has revealed its importance in maintaining cancer stem cells and contributing to mitochondrial injury, respectively. This provides potential therapeutic targets for these diseases.
References：
1. Lin, Peng, Chen, Wenyan, Long, Zhilin, Li, Lei, Xie, Qi. 2024. RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation. In Cell discovery, 10, 32. doi:10.1038/s41421-024-00654-3. https://pubmed.ncbi.nlm.nih.gov/38503731/
2. Mbita, Zukile, Hull, Rodney, Mbele, Mzwandile, Makhafola, Tshepiso, Dlamini, Zodwa. . Expression Analysis of RbBP6 in human cancers: a Prospective biomarker. In Anti-cancer drugs, 30, 767-773. doi:10.1097/CAD.0000000000000809. https://pubmed.ncbi.nlm.nih.gov/31274515/
3. Hu, Hongtu, Hu, Jijia, Chen, Zhaowei, Ding, Guohua, Liang, Wei. 2024. RBBP6-Mediated ERRα Degradation Contributes to Mitochondrial Injury in Renal Tubular Cells in Diabetic Kidney Disease. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2405153. doi:10.1002/advs.202405153. https://pubmed.ncbi.nlm.nih.gov/39441040/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen