C57BL/6JCya-Scn10aem1/Cya
Common Name:
Scn10a-KO
Product ID:
S-KO-18515
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Scn10a-KO
Strain ID
KOCMP-20264-Scn10a-B6J-VB
Gene Name
Product ID
S-KO-18515
Gene Alias
Nav1.8; PN3; SNS
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Scn10aem1/Cya mice (Catalog S-KO-18515) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000084787
NCBI RefSeq
NM_001205321
Target Region
Exon 5~6
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Scn10a, encoding the neuronal voltage-gated sodium channel NaV1.8, is involved in various physiological and pathological processes. It has been associated with pathways related to cardiac electrophysiology, pain sensation, and potentially other neurological functions. Genetic models, such as KO and CKO mouse models, have been crucial in understanding its functions [2].
In cardiac research, Scn10a-short, a transcript from an intronic enhancer-promoter complex in cardiomyocytes of the atria, sinoatrial node, and ventricular conduction system, was identified. Disrupting the mouse intronic enhancer using Nuclease technology led to reduced cardiac Scn10a-short expression, decreased cardiac sodium current in atrial cardiomyocytes, atrial conduction slowing, and arrhythmia [1].
In pain-related studies, complete loss-of-function or knockout (KO) of Scn10a in rodent models decreased sensitivity to various pain stimuli [2].
In conclusion, Scn10a plays essential roles in cardiac conduction and pain perception. The KO/CKO mouse models have significantly contributed to understanding its role in cardiac arrhythmias and pain-related diseases, providing insights into potential therapeutic targets for these conditions.
References:
1. Man, Joyce C K, Bosada, Fernanda M, Scholman, Koen T, Barnett, Phil, Christoffels, Vincent M. 2021. Variant Intronic Enhancer Controls SCN10A-short Expression and Heart Conduction. In Circulation, 144, 229-242. doi:10.1161/CIRCULATIONAHA.121.054083. https://pubmed.ncbi.nlm.nih.gov/33910361/
2. Xue, Yaping, Chidiac, Celeste, Herault, Yann, Gaveriaux-Ruff, Claire. 2021. Pain behavior in SCN9A (Nav1.7) and SCN10A (Nav1.8) mutant rodent models. In Neuroscience letters, 753, 135844. doi:10.1016/j.neulet.2021.135844. https://pubmed.ncbi.nlm.nih.gov/33775738/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen