C57BL/6JCya-Ccdc25em1/Cya
Common Name:
Ccdc25-KO
Product ID:
S-KO-18577
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ccdc25-KO
Strain ID
KOCMP-67179-Ccdc25-B6J-VB
Gene Name
Product ID
S-KO-18577
Gene Alias
2610528H13Rik; NSrp70
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccdc25em1/Cya mice (Catalog S-KO-18577) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022614
NCBI RefSeq
NM_145944
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Ccdc25, short for coiled-coil domain containing 25, is a molecule with diverse functions. It acts as a transmembrane protein and a NET-DNA receptor on cancer cells, activating the ILK-β-parvin pathway to enhance cell motility, which is crucial in cancer metastasis [1]. In the nervous system, it can affect the proliferation and differentiation of neural stem cells (NSCs) as well as neuronal maturation, likely through regulating Egr1 [2].
In cancer research, Ccdc25-knockout cells show abrogated NET-mediated metastasis, indicating its importance in the process of cancer cells being attracted by NET-DNA to form distant metastases [1]. In the context of brain development, suppression of Ccdc25 by shRNAs increases NSCs proliferation and inhibits their premature terminal mitosis and neuronal differentiation, while overexpression has the opposite effects [2].
In summary, Ccdc25 is involved in key biological processes such as cancer metastasis and neural development. Gene-knockout models have been instrumental in revealing its role in these areas, highlighting its potential as a therapeutic target for preventing cancer metastasis and understanding neural development disorders.
References：
1. Yang, Linbin, Liu, Qiang, Zhang, Xiaoqian, Su, Shicheng, Song, Erwei. 2020. DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25. In Nature, 583, 133-138. doi:10.1038/s41586-020-2394-6. https://pubmed.ncbi.nlm.nih.gov/32528174/
2. Wang, Chong, Qin, Jie, Jiao, Jianwei, Ji, Fen. 2023. Ccdc25 regulates neurogenesis during the brain development. In Developmental neurobiology, 83, 91-103. doi:10.1002/dneu.22911. https://pubmed.ncbi.nlm.nih.gov/37092777/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen