C57BL/6JCya-Gusbem1/Cya
Common Name
Gusb-KO
Product ID
S-KO-18633
Backgroud
C57BL/6JCya
Strain ID
KOCMP-110006-Gusb-B6J-VA
Status
When using this mouse strain in a publication, please cite “Gusb-KO Mouse (Catalog S-KO-18633) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gusb-KO
Strain ID
KOCMP-110006-Gusb-B6J-VA
Gene Name
Product ID
S-KO-18633
Gene Alias
g, Gur, Gus, Gut, asd, Gus-r, Gus-s, Gus-t, Gus-u
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000026613
NCBI RefSeq
NM_010368
Target Region
Exon 2~8
Size of Effective Region
~4.6 kb
Overview of Gene Research
Gusb, encoding β -glucuronidase (GUS), is essential for degrading glycosaminoglycans (GAGs), including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. Deficiency in GUSB leads to mucopolysaccharidosis VII (MPS VII; Sly syndrome), an autosomal recessive disorder associated with various symptoms such as mental retardation, short stature, and hepatosplenomegaly [3].
In an endometriosis C57BL6 mouse model, gut dysbiosis-derived GUSB promoted the development of endometriosis by enhancing the proliferation and invasion of endometrial stromal cells and causing macrophage dysfunction [1]. In hepatocellular carcinoma, over-expression of GUSB led to primary resistance of anti-PD1 therapy as it facilitated cell proliferation, invasion, and migration and downregulated PD-L1 expression by promoting miR-513a-5p [2].
In conclusion, Gusb is crucial for GAG degradation, and its dysfunction is associated with MPS VII. Studies in mouse models have also revealed its role in endometriosis and hepatocellular carcinoma, highlighting its significance in understanding these disease mechanisms.
References：
1. Wei, Yajing, Tan, Hao, Yang, Ruyu, Yao, Shuzhong, Liang, Yanchun. 2023. Gut dysbiosis-derived β-glucuronidase promotes the development of endometriosis. In Fertility and sterility, 120, 682-694. doi:10.1016/j.fertnstert.2023.03.032. https://pubmed.ncbi.nlm.nih.gov/37178109/
2. Kong, Xiangyi, Zheng, Zhiying, Song, Guoxin, Tang, Weiwei, Xia, Yongxiang. 2022. Over-Expression of GUSB Leads to Primary Resistance of Anti-PD1 Therapy in Hepatocellular Carcinoma. In Frontiers in immunology, 13, 876048. doi:10.3389/fimmu.2022.876048. https://pubmed.ncbi.nlm.nih.gov/35812439/
3. Tomatsu, Shunji, Montaño, Adriana M, Dung, Vu Chi, Grubb, Jeffrey H, Sly, William S. . Mutations and polymorphisms in GUSB gene in mucopolysaccharidosis VII (Sly Syndrome). In Human mutation, 30, 511-9. doi:10.1002/humu.20828. https://pubmed.ncbi.nlm.nih.gov/19224584/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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