C57BL/6JCya-Id3em1/Cya
Common Name:
Id3-KO
Product ID:
S-KO-18857
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Id3-KO
Strain ID
KOCMP-15903-Id3-B6J-VA
Gene Name
Product ID
S-KO-18857
Gene Alias
Hlh462; Idb3; bHLHb25
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Id3em1/Cya mice (Catalog S-KO-18857) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000008016
NCBI RefSeq
NM_008321
Target Region
Exon 1~2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Check the content of the review
Id3, also known as inhibitor of DNA-binding/differentiation-3, is a transcription regulator. It is involved in multiple biological processes such as T-cell development and differentiation, macrophage polarization, and plays a role in various diseases including cancer. In T-cell development, it transiently acts downstream of extracellular signals like TCR signaling, influencing E-protein binding to target genes, which is crucial for T-cell commitment and differentiation [2]. In macrophages, it helps control the balance between activating and inhibitory receptors, thereby affecting their anti-tumor activity [1].
In loss-and gain-of-function experiments, ID3 was shown to be sufficient to endow macrophages with potent anti-tumor activity. ID3 deficiency promoted the metastatic ability of lung cancer cells through its effects on the transcriptional regulation of CDH1, indicating its tumor-suppressive role as a downstream effector of p53 [3]. In the context of T-cell function, ID3 deficiency significantly interrupted the maintenance of tumor-specific memory CD8+ T cells in draining lymph nodes during tumorigenesis, resulting in decreased tumor-infiltrating CD8+ T cells and impaired tumor control [4].
In conclusion, Id3 is a key transcription regulator involved in immune cell function and cancer-related processes. Model-based research, especially through gene knockout experiments, has revealed its importance in maintaining the balance of immune responses and suppressing tumor development, providing insights into potential therapeutic strategies for cancer treatment.
References:
1. Deng, Zihou, Loyher, Pierre-Louis, Lazarov, Tomi, Iacobuzio-Donahue, Christine A, Geissmann, Frederic. 2024. The nuclear factor ID3 endows macrophages with a potent anti-tumour activity. In Nature, 626, 864-873. doi:10.1038/s41586-023-06950-4. https://pubmed.ncbi.nlm.nih.gov/38326607/
2. Anderson, Michele K. 2022. Shifting gears: Id3 enables recruitment of E proteins to new targets during T cell development and differentiation. In Frontiers in immunology, 13, 956156. doi:10.3389/fimmu.2022.956156. https://pubmed.ncbi.nlm.nih.gov/35983064/
3. Nagasaka, Mai, Miyajima, Chiharu, Inoue, Yasumichi, Aoyama, Mineyoshi, Hayashi, Hidetoshi. 2024. ID3 is a novel target gene of p53 and modulates lung cancer cell metastasis. In Biochemical and biophysical research communications, 708, 149789. doi:10.1016/j.bbrc.2024.149789. https://pubmed.ncbi.nlm.nih.gov/38513475/
4. Ran, Ling, Yue, Zhengliang, Ran, Mengqu, Huang, Qizhao, Xu, Lifan. 2024. The transcription regulator ID3 maintains tumor-specific memory CD8+ T cells in draining lymph nodes during tumorigenesis. In Cell reports, 43, 114690. doi:10.1016/j.celrep.2024.114690. https://pubmed.ncbi.nlm.nih.gov/39216001/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen