C57BL/6JCya-P4ha2em1/Cya
Common Name:
P4ha2-KO
Product ID:
S-KO-18924
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
P4ha2-KO
Strain ID
KOCMP-18452-P4ha2-B6J-VB
Gene Name
Product ID
S-KO-18924
Gene Alias
P4hl
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-P4ha2em1/Cya mice (Catalog S-KO-18924) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019050
NCBI RefSeq
NM_011031
Target Region
Exon 7~8
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
P4ha2, a subunit of prolyl-4-hydroxylases, contains a substrate binding and catalyzation domain and is a key enzyme in collagen synthesis [1]. It is involved in multiple biological processes and disease-related pathways, with its functions being explored through genetic models.
In chronic cholestatic liver diseases, P4HA2-/-mice showed a reduced level of ductular reaction and fibrosis compared to controls in DDC-induced chronic cholestasis, indicating that P4HA2 promotes hepatic ductular reaction and biliary fibrosis by regulating the SAV1-mediated Hippo signaling pathway [1]. In B-cell lymphoma, the absence of P4HA2 significantly impedes B lymphoma progression due to the suppression of Hh signaling in stromal fibroblasts [2].
In conclusion, P4ha2 plays a crucial role in collagen synthesis and is involved in various disease processes. The use of gene-knockout mouse models, such as P4HA2-/-mice, has been instrumental in revealing its role in chronic cholestatic liver diseases and B-cell lymphoma, providing potential therapeutic targets for these diseases.
References：
1. Zhang, Jun, Lyu, Zhuwan, Li, Bo, Xiao, Xiao, Ma, Xiong. 2023. P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases. In Hepatology (Baltimore, Md.), 78, 10-25. doi:10.1097/HEP.0000000000000317. https://pubmed.ncbi.nlm.nih.gov/36799463/
2. Li, Quanfu, Liu, Yiyang, Wu, Jingxian, Dang, Yongjun, Jiang, Wei. 2024. P4HA2 hydroxylates SUFU to regulate the paracrine Hedgehog signaling and promote B-cell lymphoma progression. In Leukemia, 38, 1751-1763. doi:10.1038/s41375-024-02313-8. https://pubmed.ncbi.nlm.nih.gov/38909089/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen