C57BL/6JCya-Pm20d1em1/Cya
Common Name
Pm20d1-KO
Product ID
S-KO-19031
Backgroud
C57BL/6JCya
Strain ID
KOCMP-212933-Pm20d1-B6J-VB
When using this mouse strain in a publication, please cite “Pm20d1-KO Mouse (Catalog S-KO-19031) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Pm20d1-KO
Strain ID
KOCMP-212933-Pm20d1-B6J-VB
Gene Name
Product ID
S-KO-19031
Gene Alias
4732466D17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000112393
NCBI RefSeq
NM_178079
Target Region
Exon 3~6
Size of Effective Region
~3.2 kb
Overview of Gene Research
Pm20d1, or Peptidase M20 Domain Containing 1, is a secreted enzyme that catalyzes the condensation of fatty acids and amino acids into bioactive lipids called N-acyl amino acids (NAAA) [3]. It is involved in multiple biological processes, with its role in metabolism and neurodegenerative diseases being of particular interest. PM20D1 is also associated with pathways like TRPV4-mediated calcium influx regulation [1]. Genetic models are valuable for studying its functions.
In a mouse model of synucleinopathy, overexpression of PM20D1 alleviated α-Syn pathology, dopaminergic neurodegeneration, and motor impairments, suggesting its protective role against Parkinson's disease progression [1]. In Alzheimer's disease, genetically increasing PM20D1 levels decreased AD-related pathologies, while decreasing its levels accelerated them, reinforcing its likely neuroprotective role [2,4]. In polycystic ovary syndrome-insulin resistance (PCOS-IR), loss of adipocyte PM20D1 aggravated insulin resistance (IR), and quercitrin, by upregulating PM20D1, corrected reproductive and metabolic abnormalities [5].
In conclusion, PM20D1 plays essential roles in metabolism-related processes and neurodegenerative diseases. Model-based research, especially through overexpression and gene-altering studies in mouse models, has revealed its protective functions in Parkinson's and Alzheimer's diseases, as well as its importance in PCOS-IR. These findings contribute to understanding the biological mechanisms of these diseases and may offer potential therapeutic targets.
References：
1. Yang, Yunying, Chen, Sichun, Zhang, Li, Yu, Zhui, Zhang, Zhentao. 2024. The PM20D1-NADA pathway protects against Parkinson's disease. In Cell death and differentiation, 31, 1545-1560. doi:10.1038/s41418-024-01356-9. https://pubmed.ncbi.nlm.nih.gov/39174646/
2. Sanchez-Mut, Jose Vicente, Glauser, Liliane, Monk, David, Gräff, Johannes. 2020. Comprehensive analysis of PM20D1 QTL in Alzheimer's disease. In Clinical epigenetics, 12, 20. doi:10.1186/s13148-020-0814-y. https://pubmed.ncbi.nlm.nih.gov/32014019/
3. Yang, Ranyao, Hu, Yue, Lee, Chi Ho, Lam, Karen S L, Xu, Aimin. 2021. PM20D1 is a circulating biomarker closely associated with obesity, insulin resistance and metabolic syndrome. In European journal of endocrinology, 186, 151-161. doi:10.1530/EJE-21-0847. https://pubmed.ncbi.nlm.nih.gov/34757919/
4. Sanchez-Mut, Jose V, Heyn, Holger, Silva, Bianca A, Esteller, Manel, Gräff, Johannes. 2018. PM20D1 is a quantitative trait locus associated with Alzheimer's disease. In Nature medicine, 24, 598-603. doi:10.1038/s41591-018-0013-y. https://pubmed.ncbi.nlm.nih.gov/29736028/
5. Li, Meihe, Gao, Shan, Kang, Minchao, Dang, Huimin, Zheng, Jin. 2023. Quercitrin alleviates lipid metabolism disorder in polycystic ovary syndrome-insulin resistance by upregulating PM20D1 in the PI3K/Akt pathway. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 117, 154908. doi:10.1016/j.phymed.2023.154908. https://pubmed.ncbi.nlm.nih.gov/37321077/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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