C57BL/6JCya-Tbpl1em1/Cya
Common Name
Tbpl1-KO
Product ID
S-KO-19163
Backgroud
C57BL/6JCya
Strain ID
KOCMP-237336-Tbpl1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Tbpl1-KO Mouse (Catalog S-KO-19163) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Tbpl1-KO
Strain ID
KOCMP-237336-Tbpl1-B6J-VA
Gene Name
Product ID
S-KO-19163
Gene Alias
STD, TLF, TRP, Tlp, TRF2, 4732475G08
Background
C57BL/6JCya
Gene Full Name
TATA box binding protein-like 1
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 10 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000127698
NCBI Transcript ID
NM_011603
Target Region
Exon 3~6
Size of Effective Region
~2.5 kb
Overview of Gene Research
Tbpl1, also known as TATA box-binding protein-like protein 1, is a transcription-related factor. It cannot bind to the TATA-box but prefers TATA-less promoters. TBPL1 shows a stronger association with TFIIA than TBP. It is required for the transcription of ribosomal protein genes containing the TCT core promoter element in Drosophila and humans. It also mediates cell cycle arrest and apoptosis [3].
In idiopathic pulmonary fibrosis (IPF) mouse models, C-MYC can activate miR-9-5p, which negatively regulates TBPL1 expression. Up-regulated C-MYC promotes pulmonary fibroblast proliferation and differentiation by inhibiting TBPL1 via miR-9-5p, triggering IPF. Inhibiting C-MYC can up-regulate TBPL1 and repress pulmonary fibrosis [1]. Fluvoxamine, a drug, alleviates bleomycin-induced lung fibrosis in mice by restraining the activation of the cGAS-STING pathway and its downstream targets including the PERK/eIF2α/c-Myc/miR-9-5p/TBPL1 signaling, attenuating fibroblast activation and migration [2].
In conclusion, TBPL1 is important in transcription regulation, especially for ribosomal protein genes. In IPF, its regulation by C-MYC and miR-9-5p plays a crucial role in the development of the disease. Mouse models have revealed its role in the context of IPF, providing insights into the disease mechanism and potential therapeutic targets.
References：
1. Qin, Hui, Tang, Yan, Mao, Yuan, Liu, Wenming, Su, Xin. 2022. C-MYC induces idiopathic pulmonary fibrosis via modulation of miR-9-5p-mediated TBPL1. In Cellular signalling, 93, 110274. doi:10.1016/j.cellsig.2022.110274. https://pubmed.ncbi.nlm.nih.gov/35122989/
2. Xie, Xiaohua, Wu, Xiaofeng, Zhao, Dongsheng, Qiu, Yan, Yang, Yungang. 2022. Fluvoxamine alleviates bleomycin-induced lung fibrosis via regulating the cGAS-STING pathway. In Pharmacological research, 187, 106577. doi:10.1016/j.phrs.2022.106577. https://pubmed.ncbi.nlm.nih.gov/36435270/
3. Mishal, Rabia, Luna-Arias, Juan Pedro. 2022. Role of the TATA-box binding protein (TBP) and associated family members in transcription regulation. In Gene, 833, 146581. doi:10.1016/j.gene.2022.146581. https://pubmed.ncbi.nlm.nih.gov/35597524/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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