C57BL/6JCya-Arel1em1/Cya
Common Name:
Arel1-KO
Product ID:
S-KO-19206
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Arel1-KO
Strain ID
KOCMP-68497-Arel1-B6J-VB
Gene Name
Product ID
S-KO-19206
Gene Alias
1110018G07Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Arel1em1/Cya mice (Catalog S-KO-19206) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043169
NCBI RefSeq
NM_178065
Target Region
Exon 5
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
AREL1, an antiapoptotic E3 ubiquitin ligase, belongs to the "Other" subfamily of HECT E3 ligases. It plays a crucial role in regulating apoptosis, necroptosis, and inflammation by ubiquitinating and degrading various proteins. It is involved in pathways such as those related to TGF-β -induced apoptosis, TNF -induced necroptosis, and IL -1β turnover [1,2,3].
In vascular endothelial cells, AREL1 is downregulated by miR-320b, and its overexpression inhibits TGF-β-induced apoptosis by downregulating SMAC, a proapoptotic protein [1]. In TNF-induced necroptosis, AREL1 ubiquitinates and promotes the degradation of MTX2, thus inhibiting necroptosis. Inhibition of AREL1-dependent ubiquitination of MTX2 could potentially sensitize tumor cells to TNF-induced necroptosis [2]. Additionally, AREL1, along with UBE2L3 and TRIP12, limits inflammation by promoting pro-IL-1β turnover [3].
In conclusion, AREL1 is essential in regulating apoptosis, necroptosis, and inflammation. Studies on its function in various disease-related processes, such as arteriosclerosis and tumor cell necroptosis, provide potential therapeutic targets for these diseases.
References：
1. Li, Yun, Song, Yunhong, Liang, Yulian. 2023. AREL1 resists the apoptosis induced by TGF-β by inhibiting SMAC in vascular endothelial cells. In Journal of biochemical and molecular toxicology, 37, e23439. doi:10.1002/jbt.23439. https://pubmed.ncbi.nlm.nih.gov/37522329/
2. Jo, Yongsam, Kim, Byeongmo, Shin, Deug Y. 2021. AREL1 E3 ubiquitin ligase inhibits TNF-induced necroptosis via the ubiquitination of MTX2. In Experimental and therapeutic medicine, 22, 1195. doi:10.3892/etm.2021.10629. https://pubmed.ncbi.nlm.nih.gov/34584540/
3. Mishra, Vishwas, Crespo-Puig, Anna, McCarthy, Callum, Morrison, Rebecca, Shenoy, Avinash R. 2023. IL-1β turnover by the UBE2L3 ubiquitin conjugating enzyme and HECT E3 ligases limits inflammation. In Nature communications, 14, 4385. doi:10.1038/s41467-023-40054-x. https://pubmed.ncbi.nlm.nih.gov/37474493/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen