C57BL/6JCya-Safbem1/Cya
Common Name:
Safb-KO
Product ID:
S-KO-19237
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Safb-KO
Strain ID
KOCMP-224903-Safb-B6J-VA
Gene Name
Product ID
S-KO-19237
Gene Alias
3110021E02Rik; 5330423C17Rik; E130307D12; HAP; HET; SAF-B1; SAFB1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Safbem1/Cya mice (Catalog S-KO-19237) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000182533
NCBI RefSeq
NM_001374619
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Safb, also known as scaffold attachment factor B, is a conserved RNA-binding protein essential for early mammalian development. It is involved in multiple biological pathways, such as gene regulation, DNA repair, and RNA processing. It has been implicated in various biological processes including cell fate determination, apoptosis, and maintaining genome integrity [1,2,4]. Genetic models like KO/CKO mouse models are valuable for studying its functions.
In mouse embryonic stem cells, knockout of Safb/Safb2 led to differential expression of around 1000 genes related to apoptosis, cell division, and cell migration, indicating its role in promoting the expression of certain genes by binding nascent RNA [1].
In adult hippocampal neural stem cells, conditional deletion of Safb increased the expression of NFIB and oligodendrocyte formation, showing that Safb blocks oligodendrocyte production by enhancing Drosha-dependent cleavage of Nfib mRNA [2].
In acute myeloid leukemia (AML), perturbation of SAFB phenocopied HOXA9 knockout, decreasing AML proliferation and increasing differentiation and apoptosis, suggesting that SAFB forms a repressive complex with HOXA9 to maintain AML [3].
In conclusion, Safb plays diverse and crucial biological functions. Through model-based research, especially KO/CKO mouse models, its role in cell-fate regulation, gene expression, and disease conditions like AML has been revealed. Understanding Safb's functions helps in further exploring biological processes and developing potential therapeutic strategies for related diseases.
References：
1. Cherney, Rachel E, Eberhard, Quinn E, Giri, Gilbert, Dominguez, Daniel, Calabrese, J Mauro. 2023. SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells. In RNA (New York, N.Y.), 29, 1535-1556. doi:10.1261/rna.079569.122. https://pubmed.ncbi.nlm.nih.gov/37468167/
2. Forcella, Pascal, Ifflander, Niklas, Rolando, Chiara, Bock, Thomas, Taylor, Verdon. 2024. SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA. In eLife, 13, . doi:10.7554/eLife.74940. https://pubmed.ncbi.nlm.nih.gov/38722021/
3. Agrawal-Singh, Shuchi, Bagri, Jaana, Giotopoulos, George, Whetton, Anthony D, Huntly, Brian J P. . HOXA9 forms a repressive complex with nuclear matrix-associated protein SAFB to maintain acute myeloid leukemia. In Blood, 141, 1737-1754. doi:10.1182/blood.2022016528. https://pubmed.ncbi.nlm.nih.gov/36577137/
4. Norman, Michael, Rivers, Caroline, Lee, Youn-Bok, Idris, Jalilah, Uney, James. . The increasing diversity of functions attributed to the SAFB family of RNA-/DNA-binding proteins. In The Biochemical journal, 473, 4271-4288. doi:. https://pubmed.ncbi.nlm.nih.gov/27888239/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen