C57BL/6JCya-Slc25a33em1/Cya
Common Name:
Slc25a33-KO
Product ID:
S-KO-19333
Background:
C57BL/6JCya
Product Type
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Genotype
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Basic Information
Strain Name
Slc25a33-KO
Strain ID
KOCMP-70556-Slc25a33-B6J-VB
Gene Name
Product ID
S-KO-19333
Gene Alias
5730438N18Rik; Pnc1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc25a33em1/Cya mice (Catalog S-KO-19333) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105686
NCBI RefSeq
NM_027460
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Slc25a33, a member of the solute carrier family 25, is a mitochondrial pyrimidine nucleotide transporter [2]. It plays a crucial role in importing/exporting pyrimidine nucleotides into and from mitochondria, which is essential for mitochondrial DNA and RNA synthesis and breakdown [2].
Deficiency of the mitochondrial protease YME1L causes inflammation in mouse retinas and cultured cells, driving the release of mtDNA and a cGAS-STING-TBK1-dependent inflammatory response [1]. This process requires SLC25A33 and is suppressed upon replenishment of cellular pyrimidine pools [1]. Overexpression of SLC25A33 is sufficient to induce immune signalling by mtDNA [1].
In conclusion, Slc25a33 is essential for maintaining pyrimidine nucleotide balance in mitochondria, which is crucial for mitochondrial genetic material synthesis and turnover [2]. Through studies related to YME1L deficiency, it has been revealed that Slc25a33 is involved in the mtDNA-dependent innate immune response, highlighting its significance in the connection between metabolism and immunity [1].
References：
1. Sprenger, Hans-Georg, MacVicar, Thomas, Bahat, Amir, Giavalisco, Patrick, Langer, Thomas. 2021. Cellular pyrimidine imbalance triggers mitochondrial DNA-dependent innate immunity. In Nature metabolism, 3, 636-650. doi:10.1038/s42255-021-00385-9. https://pubmed.ncbi.nlm.nih.gov/33903774/
2. Di Noia, Maria Antonietta, Todisco, Simona, Cirigliano, Angela, Iacobazzi, Vito, Palmieri, Ferdinando. 2014. The human SLC25A33 and SLC25A36 genes of solute carrier family 25 encode two mitochondrial pyrimidine nucleotide transporters. In The Journal of biological chemistry, 289, 33137-48. doi:10.1074/jbc.M114.610808. https://pubmed.ncbi.nlm.nih.gov/25320081/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen