C57BL/6JCya-Ndst4em1/Cya
Common Name
Ndst4-KO
Product ID
S-KO-19403
Backgroud
C57BL/6JCya
Strain ID
KOCMP-64580-Ndst4-B6J-VB
Status
When using this mouse strain in a publication, please cite “Ndst4-KO Mouse (Catalog S-KO-19403) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ndst4-KO
Strain ID
KOCMP-64580-Ndst4-B6J-VB
Gene Name
Product ID
S-KO-19403
Gene Alias
4930439H17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000173932
NCBI RefSeq
NM_022565
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Overview of Gene Research
Ndst4, a member of the glucosaminyl N-deacetylase/N-sulfotransferase (NDST) family, is involved in heparan sulfate sulfation initiation [4]. It maps to human chromosome 4q26 and may be associated with the serotonergic synapse signaling pathway, as well as having potential links to resistin level regulation [3,5].
An Ndst4 knockout (Ndst4-/-) mouse strain was generated. Ndst4-/-mice showed no gross or histological abnormalities in most organs except the colon, where there was an increased number of goblet cells, a decreased number of colonocytes in the proximal colon, and an increased basal level of apoptosis in the colonic epithelium. This indicates Ndst4's involvement in the development and homeostasis of the colonic epithelium [2]. In colorectal cancer, NDST4 is a novel candidate tumor suppressor gene. Its genetic loss was significantly associated with advanced pathological stage and poorer overall survival of patients, suggesting its role in CRC progression [1].
In conclusion, Ndst4 plays a crucial role in maintaining colonic epithelial homeostasis, likely through directing the biosynthesis of specific heparan sulfate proteoglycans. Its loss of function may contribute to the tumorigenesis and progression of colorectal cancer, as demonstrated by the Ndst4 knockout mouse model [1,2].
References：
1. Tzeng, Sheng-Tai, Tsai, Ming-Hong, Chen, Chi-Long, Yen, Sou-Jhy, Yang, Ya-Chien. 2013. NDST4 is a novel candidate tumor suppressor gene at chromosome 4q26 and its genetic loss predicts adverse prognosis in colorectal cancer. In PloS one, 8, e67040. doi:10.1371/journal.pone.0067040. https://pubmed.ncbi.nlm.nih.gov/23825612/
2. Jao, Tzu-Ming, Li, Ya-Lin, Lin, Shu-Wha, Tsai, Ming-Hong, Yang, Ya-Chien. . Alteration of colonic epithelial cell differentiation in mice deficient for glucosaminyl N-deacetylase/N-sulfotransferase 4. In Oncotarget, 7, 84938-84950. doi:10.18632/oncotarget.12915. https://pubmed.ncbi.nlm.nih.gov/27793051/
3. Qi, Qibin, Menzaghi, Claudia, Smith, Shelly, Liu, Yongmei, Qi, Lu. 2012. Genome-wide association analysis identifies TYW3/CRYZ and NDST4 loci associated with circulating resistin levels. In Human molecular genetics, 21, 4774-80. doi:10.1093/hmg/dds300. https://pubmed.ncbi.nlm.nih.gov/22843503/
4. Aikawa, J, Grobe, K, Tsujimoto, M, Esko, J D. 2000. Multiple isozymes of heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase. Structure and activity of the fourth member, NDST4. In The Journal of biological chemistry, 276, 5876-82. doi:. https://pubmed.ncbi.nlm.nih.gov/11087757/
5. Xia, Ning, Wang, Jie, Guo, Qiuting, Chang, Xing, Zhang, Xiaofei. 2024. Deciphering the antidepressant effects of Rosa damascena essential oil mediated through the serotonergic synapse signaling pathway. In Journal of ethnopharmacology, 328, 118007. doi:10.1016/j.jep.2024.118007. https://pubmed.ncbi.nlm.nih.gov/38492791/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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