C57BL/6JCya-Ap3m2em1/Cya
Common Name:
Ap3m2-KO
Product ID:
S-KO-19421
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ap3m2-KO
Strain ID
KOCMP-64933-Ap3m2-B6J-VC
Gene Name
Product ID
S-KO-19421
Gene Alias
5830445E16Rik; AP-3B
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ap3m2em1/Cya mice (Catalog S-KO-19421) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000163739
NCBI RefSeq
NM_001122820
Target Region
Exon 3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Ap3m2, also known as adaptor-related protein complex 3 subunit mu 2, is involved in protein trafficking to lysosomes pathway and is associated with specialized organelles in neuronal cells [1]. It plays a role in multiple biological processes and has implications in various diseases.
In colorectal cancer, knockdown of Ap3m2 significantly reduced the viability of CRC cell lines, and its expression was found to interact with autophagy-related genes and ROS levels, suggesting targeting it could be a strategy for CRC treatment [1].
In epilepsy research, although no missense or nonsense mutations were detected in Japanese epilepsy patients, 21 sequence variations in Ap3m2 were identified, indicating some mutations could still be candidates for neuronal disorders [2].
In alcohol-related studies, functional validation in Ap3m2 knockout mice confirmed the relationship between Ap3m2 and alcohol preference, withdrawal, and its expression was regulated by alcohol and genetics [3].
In colon cancer, bioinformatics and experimental validation showed that Ap3m2 is a cancer gene, associated with survival, could predict chemotherapy effectiveness, and might inhibit tumor growth via influencing tumor-infiltrating immune cells [4].
In conclusion, Ap3m2 is important in protein trafficking and has implications in multiple disease areas such as colorectal cancer, epilepsy, and alcohol-related behaviors. Gene knockout mouse models have been crucial in validating its role in these biological processes and disease conditions, providing insights into potential therapeutic targets and disease mechanisms.
References:
1. El Boustani, Maguie, Mouawad, Nayla, Abou Alezz, Monah. 2024. AP3M2: A key regulator from the nervous system modulates autophagy in colorectal cancer. In Tissue & cell, 91, 102593. doi:10.1016/j.tice.2024.102593. https://pubmed.ncbi.nlm.nih.gov/39488930/
2. Huang, Ming-Chih, Okada, Motohiro, Nakatsu, Fubito, Ohno, Hiroshi, Kojima, Toshio. 2007. Mutation screening of AP3M2 in Japanese epilepsy patients. In Brain & development, 29, 462-7. doi:. https://pubmed.ncbi.nlm.nih.gov/17293072/
3. Bubier, Jason A, Jay, Jeremy J, Baker, Christopher L, Crabbe, John C, Chesler, Elissa J. 2014. Identification of a QTL in Mus musculus for alcohol preference, withdrawal, and Ap3m2 expression using integrative functional genomics and precision genetics. In Genetics, 197, 1377-93. doi:10.1534/genetics.114.166165. https://pubmed.ncbi.nlm.nih.gov/24923803/
4. Jin, Qianqian, Feng, Jiahao, Yan, Yang, Kuang, Yong. 2024. Prognostic and immunological role of adaptor related protein complex 3 subunit mu2 in colon cancer. In Scientific reports, 14, 483. doi:10.1038/s41598-023-50452-2. https://pubmed.ncbi.nlm.nih.gov/38177168/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen