C57BL/6JCya-Angptl2em1/Cya
Common Name:
Angptl2-KO
Product ID:
S-KO-19435
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Angptl2-KO
Strain ID
KOCMP-26360-Angptl2-B6J-VB
Gene Name
Product ID
S-KO-19435
Gene Alias
Arp2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Angptl2em1/Cya mice (Catalog S-KO-19435) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000004208
NCBI RefSeq
NM_011923
Target Region
Exon 3
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Angptl2, or Angiopoietin-like protein 2, is a secreted glycoprotein and a member of the angiopoietin-like protein family. It has diverse functions including being involved in angiogenesis, hematopoiesis, and tumorigenesis. It exerts effects via receptors such as integrin α5β1, LILRB2, and MAG, and is associated with multiple signaling pathways like MAPKs, Akt, NF-κB, and DUSP1 pathway [2,3,5,6,7]. It is of great biological importance as it is implicated in various physiological and pathological processes [5].
In a mouse model of ICI-related autoimmune myocarditis, ANGPTL2 deficiency attenuated autoimmune inflammation, with decreased T cells and macrophages. Cardiac myofibroblast-derived ANGPTL2 enhanced chemoattractant expression via the NF-κB pathway, accelerating T cell recruitment into heart tissues [1]. In doxorubicin-induced cardiotoxicity in mice, ANGPTL2 overexpression exacerbated cardiac dysfunction, apoptosis, and oxidative stress, while its knockdown alleviated these effects through the DUSP1 pathway [3]. In acute lung injury mouse models, ANGPTL2 knockout alleviated LPS-induced pathological symptoms, reduced inflammation, and modulated alveolar macrophage polarization [4].
In conclusion, Angptl2 plays crucial roles in inflammation, cardiac function, and immune-related processes. Studies using gene knockout mouse models have revealed its significance in diseases such as autoimmune myocarditis, doxorubicin-induced cardiotoxicity, and acute lung injury, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Horiguchi, Haruki, Kadomatsu, Tsuyoshi, Yamashita, Tomoya, Miyata, Keishi, Oike, Yuichi. 2023. ANGPTL2 promotes immune checkpoint inhibitor-related murine autoimmune myocarditis. In Communications biology, 6, 965. doi:10.1038/s42003-023-05338-4. https://pubmed.ncbi.nlm.nih.gov/37736764/
2. Oike, Yuichi, Tian, Zhe, Miyata, Keishi, Endo, Motoyoshi, Kadomatsu, Tsuyoshi. 2017. ANGPTL2 - A New Causal Player in Accelerating Heart Disease Development in the Aging. In Circulation journal : official journal of the Japanese Circulation Society, 81, 1379-1385. doi:10.1253/circj.CJ-17-0854. https://pubmed.ncbi.nlm.nih.gov/28867689/
3. Liu, Cheng, Chen, Qiuling, Liu, Huadong. . ANGPTL2 aggravates doxorubicin-induced cardiotoxicity via inhibiting DUSP1 pathway. In Bioscience, biotechnology, and biochemistry, 86, 1631-1640. doi:10.1093/bbb/zbac156. https://pubmed.ncbi.nlm.nih.gov/36107816/
4. Yang, Fan, Chen, Muhu, Liu, Ying, Yu, Youwei, Deng, Lu. . ANGPTL2 knockdown induces autophagy to relieve alveolar macrophage pyroptosis by reducing LILRB2-mediated inhibition of TREM2. In Journal of cellular and molecular medicine, 28, e18280. doi:10.1111/jcmm.18280. https://pubmed.ncbi.nlm.nih.gov/38758159/
5. Takano, Mami, Hirose, Naoto, Sumi, Chikako, Asakawa, Yuki, Tanimoto, Kotaro. 2019. ANGPTL2 Promotes Inflammation via Integrin α5β1 in Chondrocytes. In Cartilage, 13, 885S-897S. doi:10.1177/1947603519878242. https://pubmed.ncbi.nlm.nih.gov/31581797/
6. Nishiyama, Sayuri, Hirose, Naoto, Yanoshita, Makoto, Asakawa-Tanne, Yuki, Tanimoto, Kotaro. 2021. ANGPTL2 Induces Synovial Inflammation via LILRB2. In Inflammation, 44, 1108-1118. doi:10.1007/s10753-020-01406-7. https://pubmed.ncbi.nlm.nih.gov/33538932/
7. Chen, Lu, Yu, Zhuo, Xie, Li, Zheng, Junke, Zhang, Yaping. 2023. ANGPTL2 binds MAG to efficiently enhance oligodendrocyte differentiation. In Cell & bioscience, 13, 42. doi:10.1186/s13578-023-00970-3. https://pubmed.ncbi.nlm.nih.gov/36855057/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen