C57BL/6JCya-Asb10em1/Cya
Common Name:
Asb10-KO
Product ID:
S-KO-19507
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Asb10-KO
Strain ID
KOCMP-117590-Asb10-B6J-VB
Gene Name
Product ID
S-KO-19507
Gene Alias
Asb-10
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asb10em1/Cya mice (Catalog S-KO-19507) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048302
NCBI RefSeq
NM_080444
Target Region
Exon 3
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Asb10, also known as Ankyrin and SOCS Box-containing protein-10, is a member of the ASB family of proteins. It has a structure with a unique N-terminus, ankyrin (ANK) repeat domains, and a suppressor of cytokine signaling (SOCS) box at the C-terminus. Asb10 functions in ubiquitin-mediated degradation pathways, where the ANK repeats bind proteins for degradation, and the SOCS box recruits ubiquitin ligase proteins to form a complex for ubiquitin transfer [2].
Evidence from multiple studies suggests its association with glaucoma. In a Pakistani cohort, certain variants in Asb10 were significantly associated with sporadic primary open-angle glaucoma (POAG), indicating it may act as a risk factor [4]. However, in a Korean cohort, no significant association was found between Asb10 gene polymorphisms and normal-tension glaucoma (NTG) [3]. Additionally, in triple-negative breast cancer, Asb10, as an estrogen receptor ERα trans-activated E3 ligase, ubiquitylates tumor endothelial marker 8 (TEM8) for degradation. Its deficiency in TNBC leads to a high homeostatic level of TEM8, which is involved in promoting stemness and vasculogenic mimicry capacity of breast cancer cells [1].
In conclusion, Asb10 plays a crucial role in ubiquitin-mediated protein degradation pathways. Its variants are associated with glaucoma in some ethnic populations, and it also has an impact on the development of triple-negative breast cancer by regulating the degradation of key proteins. The study of Asb10 in these disease models helps to understand the molecular mechanisms underlying these diseases and may provide potential targets for treatment.
References:
1. Xu, Jiahui, Yang, Xiaoli, Deng, Qiaodan, Zhang, Lixing, Liu, Suling. 2021. TEM8 marks neovasculogenic tumor-initiating cells in triple-negative breast cancer. In Nature communications, 12, 4413. doi:10.1038/s41467-021-24703-7. https://pubmed.ncbi.nlm.nih.gov/34285210/
2. Keller, Kate E, Wirtz, Mary K. 2016. Working your SOCS off: The role of ASB10 and protein degradation pathways in glaucoma. In Experimental eye research, 158, 154-160. doi:10.1016/j.exer.2016.06.003. https://pubmed.ncbi.nlm.nih.gov/27296073/
3. Jung, Seung-Hyun, Lee, Young Chun, Lee, Mee Yon, Shin, Hye-Young. 2019. Lack of Correlation between ASB10 and Normal-tension Glaucoma in a Population from the Republic of Korea. In Current eye research, 45, 521-525. doi:10.1080/02713683.2019.1668949. https://pubmed.ncbi.nlm.nih.gov/31522561/
4. Micheal, Shazia, Ayub, Humaira, Islam, Farrah, Khan, Muhammad Imran, den Hollander, Anneke I. 2015. Variants in the ASB10 Gene Are Associated with Primary Open Angle Glaucoma. In PloS one, 10, e0145005. doi:10.1371/journal.pone.0145005. https://pubmed.ncbi.nlm.nih.gov/26713451/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen