C57BL/6JCya-Dbtem1/Cya
Common Name
Dbt-KO
Product ID
S-KO-19828
Backgroud
C57BL/6JCya
Strain ID
KOCMP-13171-Dbt-B6J-VA
When using this mouse strain in a publication, please cite “Dbt-KO Mouse (Catalog S-KO-19828) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Dbt-KO
Strain ID
KOCMP-13171-Dbt-B6J-VA
Gene Name
Product ID
S-KO-19828
Gene Alias
BCKAD E2, BCKAD-E2, D3Wsu60e
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000000349
NCBI RefSeq
NM_010022
Target Region
Exon 4~5
Size of Effective Region
~2.6 kb
Overview of Gene Research
Dbt, as an important kinase, plays a central role in both circadian rhythms and development [1]. It is involved in the regulation of sleep, which is subject to both circadian and homeostatic regulation. The circadian rhythm determines the daily alternation of the sleep-wake cycle, and Dbt is implicated in this process.
In a study on Drosophila, ethyl methanesulfonate-induced dbt mutants were investigated for their sleep patterns [1]. Knock-down of DBT in all neurons using CRISPR-Cas9 led to elevated sleep during the day and reduced sleep at night. Reduction of dbt in circadian neurons produced less sleep at night, while lower expression in non-circadian neurons led to increased sleep during the day. The PER protein was shown to be involved in DBT-mediated sleep regulation.
In conclusion, Dbt is a crucial kinase for regulating sleep patterns through its effects on both circadian and non-circadian neurons. The use of genetic models like the Drosophila mutants in this study has provided insights into the role of Dbt in sleep-related biological processes.
References：
1. Wang, Jialin, Fan, Jin-Yuan, Zhao, Zhangwu, Dissel, Stephane, Price, Jeffrey. 2022. DBT affects sleep in both circadian and non-circadian neurons. In PLoS genetics, 18, e1010035. doi:10.1371/journal.pgen.1010035. https://pubmed.ncbi.nlm.nih.gov/35139068/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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