C57BL/6JCya-Nr3c1em1/Cya
Common Name:
Nr3c1-KO
Product ID:
S-KO-20257
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nr3c1-KO
Strain ID
KOCMP-14815-Nr3c1-B6J-VA
Gene Name
Product ID
S-KO-20257
Gene Alias
GR; Grl-1; Grl1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nr3c1em1/Cya mice (Catalog S-KO-20257) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115571
NCBI RefSeq
NM_001361210
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Nr3c1, also known as the glucocorticoid receptor gene, encodes the glucocorticoid receptor which functions as both a transcription factor and an RNA-binding protein. It is a key component of the stress-response system and is involved in multiple biological processes by regulating the hypothalamus-pituitary-adrenal (HPA) axis. Genetic models, such as KO/CKO mouse models, have been crucial in studying its functions.
Knockdown of Nr3c1 in clear cell renal cell carcinoma (ccRCC) cells inhibits their proliferation and migration through activating endoplasmic reticulum stress-mitophagy via the ATF6-PINK1/BNIP3 pathway [2]. In pancreatic β-cells, activation of Nr3c1 promotes autophagy overload under glucolipotoxic conditions. The NR3C1-FTO-m6A modifications-Atg genes axis is a key mediator of balanced autophagic flux, and inhibition of FTO can prevent excessive autophagy activation, which may offer a new therapeutic target for diabetes [1].
In summary, Nr3c1 is essential in regulating stress-related biological processes and is implicated in diseases like diabetes and ccRCC. Studies using KO/CKO mouse models have revealed its role in these disease conditions, highlighting its potential as a therapeutic target.
References：
1. Wu, Tijun, Shao, Yixue, Li, Xirui, Chen, Fang, Han, Xiao. 2023. NR3C1/Glucocorticoid receptor activation promotes pancreatic β-cell autophagy overload in response to glucolipotoxicity. In Autophagy, 19, 2538-2557. doi:10.1080/15548627.2023.2200625. https://pubmed.ncbi.nlm.nih.gov/37039556/
2. Yan, Minbo, Wang, Jinhua, Wang, Haojie, Tang, Yuxin, Dai, Yingbo. 2023. Knockdown of NR3C1 inhibits the proliferation and migration of clear cell renal cell carcinoma through activating endoplasmic reticulum stress-mitophagy. In Journal of translational medicine, 21, 701. doi:10.1186/s12967-023-04560-2. https://pubmed.ncbi.nlm.nih.gov/37807060/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen