C57BL/6JCya-Fkbp11em1/Cya
Common Name:
Fkbp11-KO
Product ID:
S-KO-20306
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fkbp11-KO
Strain ID
KOCMP-66120-Fkbp11-B6J-VA
Gene Name
Product ID
S-KO-20306
Gene Alias
1110002O23Rik; FKBP-11
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fkbp11em1/Cya mice (Catalog S-KO-20306) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003445
NCBI RefSeq
NM_024169
Target Region
Exon 1~5
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Fkbp11, also known as FK506-binding protein 11, is a member of the peptidyl-prolyl cis-trans isomerase family. It is involved in the unfolded protein response (UPR) pathway and acts as a translocon accessory factor during the synthesis of soluble secretory and transmembrane proteins at the endoplasmic reticulum (ER) [6]. It also has connections to various biological processes such as cell growth, proliferation, and apoptosis, making it biologically important in understanding disease mechanisms.
In obese mice, restoring hepatic Fkbp11 expression initiates an atypical UPR signaling pathway. It rewires PERK signaling toward NRF2, away from the eIF2α-ATF4 axis of the UPR, establishing glucose homeostasis without changing hepatic ER stress, food consumption, or body weight. This indicates that Fkbp11 could be a potential target to treat type 2 diabetes and obesity related to ER stress [1].
In hepatocellular carcinoma (HCC), Fkbp11 is upregulated, and knockdown of Fkbp11 in HCC cells attenuates proliferation and migration, suggesting its role in HCC pathogenesis. Its levels are significantly reduced post-surgery, making it a potential diagnostic and prognostic marker for HCC [2].
In osteosarcoma, Fkbp11 is highly expressed, and silencing its expression suppresses cell invasion, migration, slows proliferation, and promotes apoptosis through the MAPK pathway [3].
In oral squamous cell carcinoma (OSCC), FKBP11 promotes cell proliferation by regulating the G2/M phase and apoptosis via p53-related pathways [4].
In Crohn's disease, increased FKBP11 expression in intestinal inflammatory tissues protects intestinal epithelial cells against inflammation-induced apoptosis via the JNK-caspase pathway [5].
In conclusion, Fkbp11 plays crucial roles in multiple biological processes and disease conditions. Model-based research, especially using gene knockout or conditional knockout mouse models, has revealed its significance in diseases like type 2 diabetes, obesity, and various cancers. Understanding Fkbp11's functions provides potential targets for developing novel therapeutic strategies for these diseases.
References：
1. Herrema, Hilde, Guan, Dongxian, Choi, Jae Won, Chun, Hyonho, Ozcan, Umut. . FKBP11 rewires UPR signaling to promote glucose homeostasis in type 2 diabetes and obesity. In Cell metabolism, 34, 1004-1022.e8. doi:10.1016/j.cmet.2022.06.007. https://pubmed.ncbi.nlm.nih.gov/35793654/
2. Thokerunga, Erick, Huang, Fangfang, Bongolo, Christian Cedric, Akankwatsa, Gilbert, Tu, Jian-Cheng. . FKBP11 upregulation promotes proliferation and migration in hepatocellular carcinoma. In Cancer biomarkers : section A of Disease markers, 37, 109-120. doi:10.3233/CBM-220440. https://pubmed.ncbi.nlm.nih.gov/37248890/
3. Zeng, Duo, Li, Jiayu, Yuan, Xuhui, Lv, Xiao-Bin, Liao, Qi. 2023. FKBP11 improves the malignant property of osteosarcoma cells and acts as a prognostic factor of osteosarcoma. In Aging, 15, 2450-2459. doi:10.18632/aging.204523. https://pubmed.ncbi.nlm.nih.gov/37014329/
4. Qiu, Lin, Liu, Han, Wang, Shuang, Wang, Guan-Hua, Zhang, Jun. 2021. FKBP11 promotes cell proliferation and tumorigenesis via p53-related pathways in oral squamous cell carcinoma. In Biochemical and biophysical research communications, 559, 183-190. doi:10.1016/j.bbrc.2021.04.096. https://pubmed.ncbi.nlm.nih.gov/33945996/
5. Wang, Xiaotong, Cui, Xiaopeng, Zhu, Chuanwu, Zhang, Dongmei, Zhou, Guoxiong. 2018. FKBP11 protects intestinal epithelial cells against inflammation‑induced apoptosis via the JNK‑caspase pathway in Crohn's disease. In Molecular medicine reports, 18, 4428-4438. doi:10.3892/mmr.2018.9485. https://pubmed.ncbi.nlm.nih.gov/30221722/
6. DiGuilio, Amanda, Cheng, Ben, Zhong, Frank, Coon, Joshua J, Keenan, Robert J. 2024. The prolyl isomerase FKBP11 is a secretory translocon accessory factor. In Molecular biology of the cell, 35, ar135. doi:10.1091/mbc.E24-07-0305. https://pubmed.ncbi.nlm.nih.gov/39259761/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen