C57BL/6JCya-Csf1em1/Cya
Common Name
Csf1-KO
Product ID
S-KO-20366
Backgroud
C57BL/6JCya
Strain ID
KOCMP-12977-Csf1-B6J-VB
Status
When using this mouse strain in a publication, please cite “Csf1-KO Mouse (Catalog S-KO-20366) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Csf1-KO
Strain ID
KOCMP-12977-Csf1-B6J-VB
Gene Name
Product ID
S-KO-20366
Gene Alias
op, Csfm, MCSF, BAP025, PG-M-CSF, Mhdabap25
Background
C57BL/6JCya
Gene Full Name
colony stimulating factor 1 (macrophage)
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 3 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000118593
NCBI Transcript ID
NM_001113529
Target Region
Exon 4~6
Size of Effective Region
~6.9 kb
Overview of Gene Research
Csf1, also known as macrophage colony-stimulating factor 1, is the primary growth factor essential for the control of monocyte and macrophage differentiation, survival, proliferation and renewal [3]. It plays a crucial role in the CSF1/CSF1R signaling pathway, which is involved in many biological processes such as bone remodeling, hematopoiesis, and tumor microenvironment regulation [2,1].
In a mouse model, depletion of Csf1 in marrow adipogenic precursors (MALPs) using AdipoqCre (Csf1 CKOAdipoq mice) led to increased femoral trabecular bone mass, diminished osteoclasts in the femoral secondary spongiosa region, and reduced bone marrow cellularity, hematopoietic progenitors, and macrophages, demonstrating that MALPs-synthesized Csf1 controls bone remodeling and hematopoiesis [2]. In the context of tumors, inhibitors of the CSF1/CSF1R signaling pathway, like pexidartinib (PLX3397), can reprogram tumor-associated macrophages (TAMs), stimulate T-cell infiltration, and suppress primary tumor growth and lung metastasis in an osteosarcoma orthotopic xenograft model [1].
In conclusion, Csf1 is vital for the development, homeostasis, and tissue repair related to monocyte and macrophage functions. Gene-knockout (KO) and conditional-knockout (CKO) mouse models have been instrumental in revealing its role in bone-related diseases and cancer. Understanding Csf1's functions through these models provides potential therapeutic targets for treating diseases associated with abnormal macrophage regulation and bone metabolism.
References：
1. Fujiwara, Tomohiro, Yakoub, Mohamed A, Chandler, Andrew, Purdue, Ed, Healey, John H. 2021. CSF1/CSF1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-cell Infiltration in the Sarcoma Microenvironment. In Molecular cancer therapeutics, 20, 1388-1399. doi:10.1158/1535-7163.MCT-20-0591. https://pubmed.ncbi.nlm.nih.gov/34088832/
2. Zhong, Leilei, Lu, Jiawei, Fang, Jiankang, Dyment, Nathanial, Qin, Ling. 2023. Csf1 from marrow adipogenic precursors is required for osteoclast formation and hematopoiesis in bone. In eLife, 12, . doi:10.7554/eLife.82112. https://pubmed.ncbi.nlm.nih.gov/36779854/
3. Sehgal, Anuj, Irvine, Katharine M, Hume, David A. 2021. Functions of macrophage colony-stimulating factor (CSF1) in development, homeostasis, and tissue repair. In Seminars in immunology, 54, 101509. doi:10.1016/j.smim.2021.101509. https://pubmed.ncbi.nlm.nih.gov/34742624/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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