C57BL/6JCya-Wnt3aem1/Cya
Common Name:
Wnt3a-KO
Product ID:
S-KO-20476
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Wnt3a-KO
Strain ID
KOCMP-22416-Wnt3a-B6J-VB
Gene Name
Product ID
S-KO-20476
Gene Alias
Wnt-3a; vt
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wnt3aem1/Cya mice (Catalog S-KO-20476) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000010044
NCBI RefSeq
NM_009522
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Wnt3a, a member of the Wnt family of secreted glycoproteins, is crucial in regulating multiple cellular functions such as self-renewal, proliferation, differentiation, and motility. It exerts its effects mainly through the canonical Wnt/β-catenin signaling pathway, which is involved in numerous biological processes including development, tissue homeostasis, and disease [3].
In lung injury, Wnt3a-loaded extracellular vesicles (EVs) can promote alveolar epithelial regeneration. The engineered Wnt3aWG EVs activate Wnt signaling, enhance cell growth after human alveolar epithelial cell injury, and restore impaired pulmonary function in an elastase-induced emphysema model [1]. In colorectal cancer, Wnt3a is associated with angiogenesis and cancer progression, making it a promising target for treatment [2]. Regarding cartilage repair, WNT3a-loaded exosomes can penetrate cartilage, activate canonical WNT signaling, and improve the repair of osteochondral defects in mice, while recombinant WNT3a fails to activate the signaling in cartilage [4]. In the context of osteoarthritis, knockdown of Wnt3a in rat chondrocytes promotes collagen type II expression, suggesting it could be a novel therapy method [5]. In cardiac hypertrophy, TGFβ1 can upregulate Wnt3a expression in a Smad-dependent manner, and there is crosstalk between TGFβ1 and the canonical Wnt/β-catenin pathways [6]. Also, in hepatocarcinogenesis, the overexpression of Wnt3a in a rat model was associated with the process, and it could be a promising biomarker for early-stage HCC monitoring [7].
In conclusion, Wnt3a plays diverse and significant roles in various biological processes and disease conditions. Studies using different animal models, such as the mouse models in lung injury, cartilage repair, and cardiac hypertrophy, have revealed its functions in tissue regeneration, disease progression, and potential as a biomarker or therapeutic target. These findings contribute to a better understanding of the underlying mechanisms of related diseases and provide new perspectives for treatment strategies.
References：
1. Gao, Lei, Sun, Yongping, Zhang, Xinye, Cheng, Linzhao, Liu, Senquan. 2023. Wnt3a-Loaded Extracellular Vesicles Promote Alveolar Epithelial Regeneration after Lung Injury. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2206606. doi:10.1002/advs.202206606. https://pubmed.ncbi.nlm.nih.gov/37072558/
2. Tufail, Muhammad, Wu, Changxin. 2023. Wnt3a is a promising target in colorectal cancer. In Medical oncology (Northwood, London, England), 40, 86. doi:10.1007/s12032-023-01958-2. https://pubmed.ncbi.nlm.nih.gov/36719558/
3. He, Sha, Lu, Yi, Liu, Xia, Qian, Chao-Nan, Zhang, Jian. 2015. Wnt3a: functions and implications in cancer. In Chinese journal of cancer, 34, 554-62. doi:10.1186/s40880-015-0052-4. https://pubmed.ncbi.nlm.nih.gov/26369691/
4. Thomas, Bethan L, Eldridge, Suzanne E, Nosrati, Babak, Jupp, Ray, Dell'Accio, Francesco. 2021. WNT3A-loaded exosomes enable cartilage repair. In Journal of extracellular vesicles, 10, e12088. doi:10.1002/jev2.12088. https://pubmed.ncbi.nlm.nih.gov/34025953/
5. Shi, Shiping, Man, Zhentao, Sun, Shui. 2022. Wnt3a knockdown promotes collagen type II expression in rat chondrocytes. In Experimental and therapeutic medicine, 24, 526. doi:10.3892/etm.2022.11453. https://pubmed.ncbi.nlm.nih.gov/35837029/
6. Li, Tao, Weng, Xiaofei, Cheng, Siya, Gao, Hai, Li, Yanming. 2020. Wnt3a upregulation is involved in TGFβ1-induced cardiac hypertrophy. In Cytokine, 138, 155376. doi:10.1016/j.cyto.2020.155376. https://pubmed.ncbi.nlm.nih.gov/33243628/
7. Yao, Min, Wang, Jian-Jun, Chen, Xi-Yu, Wang, Li, Yao, Deng-Fu. 2022. Oncogenic Wnt3a is a promising sensitive biomarker for monitoring hepatocarcinogenesis. In Hepatobiliary & pancreatic diseases international : HBPD INT, 22, 263-269. doi:10.1016/j.hbpd.2022.11.004. https://pubmed.ncbi.nlm.nih.gov/36435702/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen