C57BL/6JCya-Nuak1em1flox/Cya
Common Name
Nuak1-flox
Product ID
S-CKO-16752
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-77976-Nuak1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Nuak1-flox Mouse (Catalog S-CKO-16752) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Nuak1-flox
Strain ID
CKOCMP-77976-Nuak1-B6J-VA
Gene Name
Product ID
S-CKO-16752
Gene Alias
Omphk1, B230104P22Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 10 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000020220
NCBI Transcript ID
NM_001004363
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Overview of Gene Research
NUAK1, also known as NUAK family kinase 1, is an AMP-activated protein kinase (AMPK)-related kinase. It plays a crucial role in multiple biological processes. NUAK1 is involved in regulating various signaling pathways, such as TGF-β, mTORC2/Akt, and is associated with processes like cell proliferation, apoptosis, differentiation, and migration. Aberrant NUAK1 signaling is implicated in diseases including fibrosis, cancer, and metabolic-associated steatohepatitis [2,3,4,5].
In fibrosis, fibroblast-specific loss of NUAK1 expression in mouse models of kidney, lung, and liver fibrosis significantly attenuated fibrosis. This indicates that NUAK1 promotes organ fibrosis via YAP and TGF-β/SMAD signaling, and there is a positive feedback loop with YAP and TAZ in the fibrogenic process [1].
In gastric cancer, NUAK1 depletion or inhibition in patient-derived xenograft and transgenic mouse models dramatically ameliorated gastric tumorigenesis, as it activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drive chemoresistance [3].
In metabolic-dysfunction-associated steatohepatitis (MASH), NUAK1 inhibition in MASH mouse models ameliorated steatohepatitis development by down-regulating hepatic steatosis and fibrosis, as NUAK1 activates Caspase 6-driven pyroptosis and inflammation [5].
In conclusion, NUAK1 is a key regulator in multiple biological processes and diseases. Gene-knockout or conditional-knockout mouse models have been instrumental in revealing its role in fibrosis, gastric cancer, and MASH. These findings suggest that NUAK1 could be a potential therapeutic target for treating these diseases.
References：
1. Zhang, Tianzhou, He, Xiaolin, Caldwell, Lauren, Wrana, Jeffrey L, Yuen, Darren A. 2022. NUAK1 promotes organ fibrosis via YAP and TGF-β/SMAD signaling. In Science translational medicine, 14, eaaz4028. doi:10.1126/scitranslmed.aaz4028. https://pubmed.ncbi.nlm.nih.gov/35320001/
2. van de Vis, Reinofke A J, Moustakas, Aristidis, van der Heide, Lars P. 2021. NUAK1 and NUAK2 Fine-Tune TGF-β Signaling. In Cancers, 13, . doi:10.3390/cancers13133377. https://pubmed.ncbi.nlm.nih.gov/34282782/
3. Cao, Longlong, Lin, Guangtan, Fan, Denghui, Huang, Changming, Xie, Jianwei. 2024. NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer. In Cell reports, 43, 114446. doi:10.1016/j.celrep.2024.114446. https://pubmed.ncbi.nlm.nih.gov/38996065/
4. Palma, Mario, Riffo, Elizabeth, Farias, Alejandro, Pincheira, Roxana, Castro, Ariel F. 2023. NUAK1 coordinates growth factor-dependent activation of mTORC2 and Akt signaling. In Cell & bioscience, 13, 232. doi:10.1186/s13578-023-01185-2. https://pubmed.ncbi.nlm.nih.gov/38135881/
5. Sheng, Mingwei, Huo, Shuhan, Jia, Lili, Lin, Yuanbang, Yu, Wenli. 2024. NUAK1 promotes metabolic dysfunction-associated steatohepatitis progression by activating Caspase 6-driven pyroptosis and inflammation. In Hepatology communications, 8, . doi:10.1097/HC9.0000000000000479. https://pubmed.ncbi.nlm.nih.gov/38967580/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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