C57BL/6JCya-Dnajb1em1flox/Cya
Common Name
Dnajb1-flox
Product ID
S-CKO-17011
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-81489-Dnajb1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Dnajb1-flox Mouse (Catalog S-CKO-17011) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Dnajb1-flox
Strain ID
CKOCMP-81489-Dnajb1-B6J-VA
Gene Name
Product ID
S-CKO-17011
Gene Alias
DjB1, Hdj1, HSPF1, Hsp40, 0610007I11Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 8 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000005620
NCBI Transcript ID
NM_018808
Target Region
Exon 2~3
Size of Effective Region
~1.3 kb
Overview of Gene Research
Dnajb1, also known as DnaJ homolog subfamily B member 1, is involved in multiple cellular processes. It has been shown to negatively regulate MIG6, a tumor suppressor, promoting epidermal growth factor receptor (EGFR) signaling [6]. Additionally, it destabilizes PDCD5, suppressing p53-mediated apoptosis [7].
In the context of fibrolamellar hepatocellular carcinoma (FLC), Dnajb1 forms a fusion gene with PRKACA (DNAJB1-PRKACA). This fusion transcript is the oncogenic driver in FLC. Peptide-based immunotherapy targeting the DNAJB1-PRKACA fusion protein can induce multifunctional T cells, leading to durable relapse-free survival in a patient [1]. Endogenous CD8 T cell responses to this fusion in FLC patients are rare, but functional fusion-specific T cell receptors (TCRs) have been defined, with one showing strong anti-tumor activity in vivo [2]. The DNAJB1-PRKACA fusion drives FLC through impaired SIK signaling and CRTC2/p300-mediated transcriptional reprogramming [3]. It also regulates LINC00473, which promotes tumor growth and alters mitochondrial fitness in FLC [4,5].
In summary, Dnajb1 has important roles in regulating cell signaling pathways related to tumor suppression and apoptosis. In FLC, the DNAJB1-PRKACA fusion is a key oncogenic driver, and research on this fusion provides insights into the disease mechanism and potential immunotherapeutic strategies. The study of Dnajb1 in the context of FLC using various models helps to understand the disease pathogenesis and develop new treatment options.
References：
1. Bauer, Jens, Köhler, Natalie, Maringer, Yacine, Hailfinger, Stephan, Walz, Juliane S. 2022. The oncogenic fusion protein DNAJB1-PRKACA can be specifically targeted by peptide-based immunotherapy in fibrolamellar hepatocellular carcinoma. In Nature communications, 13, 6401. doi:10.1038/s41467-022-33746-3. https://pubmed.ncbi.nlm.nih.gov/36302754/
2. Kirk, Allison M, Crawford, Jeremy Chase, Chou, Ching-Heng, Strome, Scott E, Thomas, Paul G. . DNAJB1-PRKACA fusion neoantigens elicit rare endogenous T cell responses that potentiate cell therapy for fibrolamellar carcinoma. In Cell reports. Medicine, 5, 101469. doi:10.1016/j.xcrm.2024.101469. https://pubmed.ncbi.nlm.nih.gov/38508137/
3. Gritti, Ilaria, Wan, Jinkai, Weeresekara, Vajira, Gujral, Taranjit S, Bardeesy, Nabeel. . DNAJB1-PRKACA Fusion Drives Fibrolamellar Liver Cancer through Impaired SIK Signaling and CRTC2/p300-Mediated Transcriptional Reprogramming. In Cancer discovery, 15, 382-400. doi:10.1158/2159-8290.CD-24-0634. https://pubmed.ncbi.nlm.nih.gov/39326063/
4. Ma, Rosanna K, Tsai, Pei-Yin, Farghli, Alaa R, Barrow, Joeva, Sethupathy, Praveen. 2024. DNAJB1-PRKACA fusion protein-regulated LINC00473 promotes tumor growth and alters mitochondrial fitness in fibrolamellar carcinoma. In PLoS genetics, 20, e1011216. doi:10.1371/journal.pgen.1011216. https://pubmed.ncbi.nlm.nih.gov/38512964/
5. Kim, Stephanie S, Kycia, Ina, Karski, Michael, Sethupathy, Praveen, Vakili, Khashayar. 2022. DNAJB1-PRKACA in HEK293T cells induces LINC00473 overexpression that depends on PKA signaling. In PloS one, 17, e0263829. doi:10.1371/journal.pone.0263829. https://pubmed.ncbi.nlm.nih.gov/35167623/
6. Park, Soo-Yeon, Choi, Hyo-Kyoung, Seo, Jae Sung, Choi, Kyung-Chul, Yoon, Ho-Geun. 2015. DNAJB1 negatively regulates MIG6 to promote epidermal growth factor receptor signaling. In Biochimica et biophysica acta, 1853, 2722-30. doi:10.1016/j.bbamcr.2015.07.024. https://pubmed.ncbi.nlm.nih.gov/26239118/
7. Cui, Xiandan, Choi, Hyo-Kyoung, Choi, Young-Seok, Choi, Kyung-Chul, Yoon, Ho-Geun. 2014. DNAJB1 destabilizes PDCD5 to suppress p53-mediated apoptosis. In Cancer letters, 357, 307-315. doi:10.1016/j.canlet.2014.11.041. https://pubmed.ncbi.nlm.nih.gov/25444898/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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