C57BL/6JCya-Unc5bem1flox/Cya
Common Name
Unc5b-flox
Product ID
S-CKO-18061
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-107449-Unc5b-B6J-VB
Status
When using this mouse strain in a publication, please cite “Unc5b-flox Mouse (Catalog S-CKO-18061) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Unc5b-flox
Strain ID
CKOCMP-107449-Unc5b-B6J-VB
Gene Name
Product ID
S-CKO-18061
Gene Alias
Unc5h2, A630020F16, D10Bwg0792e, 6330415E02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 10 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000077925
NCBI Transcript ID
NM_029770.3
Target Region
Exon 5
Size of Effective Region
~0.7 kb
Overview of Gene Research
Unc5b, an axon guidance regulator, is also expressed in various cell types and is involved in multiple biological processes. It is a receptor for Netrin-1 and is associated with pathways such as Wnt/β-catenin signaling. Unc5b plays a crucial role in maintaining the integrity of barriers like the blood-brain barrier (BBB) and blood-retina barrier (BRB), and is also implicated in processes such as neural differentiation, angiogenesis, and macrophage function in the context of diseases [1,3].
In in vivo studies, inducible endothelial-specific deletion of Unc5B in adult mice led to BBB leak, with changes in barrier-related gene expression and a decrease in Wnt/β-catenin signaling, indicating its role in maintaining BBB integrity [1]. Conditional deletion of Unc5b in monocytes and macrophages of mice with advanced atherosclerosis resulted in a decrease in atherosclerotic plaque burden, reduced plaque complexity, and improved macrophage efferocytotic capacity, suggesting it as a therapeutic target for atherosclerosis [2].
In conclusion, Unc5b is essential for maintaining the integrity of neurovascular barriers and has a significant impact on macrophage-related processes in atherosclerosis. The use of gene knockout and conditional knockout mouse models has revealed its critical functions in these disease-relevant biological processes, providing potential therapeutic targets for CNS diseases and atherosclerosis.
References：
1. Boyé, Kevin, Geraldo, Luiz Henrique, Furtado, Jessica, Ackerman, Susan L, Eichmann, Anne. 2022. Endothelial Unc5B controls blood-brain barrier integrity. In Nature communications, 13, 1169. doi:10.1038/s41467-022-28785-9. https://pubmed.ncbi.nlm.nih.gov/35246514/
2. Schlegel, Martin, Cyr, Yannick, Newman, Alexandra A C, Fisher, Edward A, Moore, Kathryn J. 2024. Targeting Unc5b in macrophages drives atherosclerosis regression and pro-resolving immune cell function. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2412690121. doi:10.1073/pnas.2412690121. https://pubmed.ncbi.nlm.nih.gov/39436659/
3. Furtado, Jessica, Geraldo, Luiz Henrique, Leser, Felipe Saceanu, Eichmann, Anne, Boyé, Kevin. 2023. Netrin-1 binding to Unc5B regulates Blood-Retina Barrier integrity. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.01.21.525006. https://pubmed.ncbi.nlm.nih.gov/36711611/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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