C57BL/6JCya-Fkbp5em1/Cya
Common Name
Fkbp5-KO
Product ID
S-KO-02069
Backgroud
C57BL/6JCya
Strain ID
KOCMP-14229-Fkbp5-B6J-VA
Status
When using this mouse strain in a publication, please cite “Fkbp5-KO Mouse (Catalog S-KO-02069) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Fkbp5-KO
Strain ID
KOCMP-14229-Fkbp5-B6J-VA
Gene Name
Product ID
S-KO-02069
Gene Alias
D17Ertd592e, Dit1, FKBP-5, FKBP51
Background
C57BL/6JCya
Gene Full Name
--
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 17 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000079413
NCBI Transcript ID
NM_010220
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Overview of Gene Research
FKBP5, also known as FKBP51, encodes a co-chaperone protein. It is involved in modulating glucocorticoid receptor interaction, thus playing a crucial role in the adaptive stress response. The intracellular concentration of FKBP5 affects the binding affinity of the glucocorticoid receptor to glucocorticoids. The gene contains glucocorticoid response elements in its introns which regulate its expression [2].
In a cardiomyocyte-specific FKBP5 knockdown mouse model, downregulation of FKBP5 promoted atrial arrhythmogenesis. This was associated with action potential alternans, spontaneous Ca2+ waves, and increased NCX1 protein levels and activity. FKBP5-deficiency enhanced Slc8a1 (encoding NCX1) transcription via hypoxia-inducible factor 1α, as FKBP5 negatively modulates hypoxia-inducible factor 1α protein levels by interacting with heat-shock protein 90 [1]. In Fkbp5 knockout mice, there was no significant change in circadian rhythms detected by wheel-running activity, but it offered protection against stress-mediated disruptions in rhythmicity in a sex-dependent manner. Also, protein changes were observed in a brain region-and sex-dependent manner, with elevated levels of proteins associated with neuronal-cell adhesion and synaptic integrity in aged Fkbp5 KOs [3].
In conclusion, FKBP5 is essential for regulating the adaptive stress response by modulating glucocorticoid receptor interaction. The use of FKBP5 knockout or knockdown mouse models has revealed its role in atrial arrhythmogenesis and its impact on circadian rhythm and brain proteomics. These findings contribute to our understanding of related disease mechanisms, such as those in atrial fibrillation and potentially stress-related neuropsychiatric disorders [1,3].
References:
1. Wang, Xiaolei, Song, Jia, Yuan, Yue, Dobrev, Dobromir, Li, Na. 2023. Downregulation of FKBP5 Promotes Atrial Arrhythmogenesis. In Circulation research, 133, e1-e16. doi:10.1161/CIRCRESAHA.122.322213. https://pubmed.ncbi.nlm.nih.gov/37154033/
2. Mendonça, Mariana S, Mangiavacchi, Paula M, Rios, Álvaro F L. 2021. Regulatory functions of FKBP5 intronic regions associated with psychiatric disorders. In Journal of psychiatric research, 143, 1-8. doi:10.1016/j.jpsychires.2021.08.014. https://pubmed.ncbi.nlm.nih.gov/34433110/
3. Gebru, Niat T, Guergues, Jennifer, Verdina, Laura A, Gulick, Danielle, Blair, Laura J. 2024. Fkbp5 gene deletion: Circadian rhythm profile and brain proteomics in aged mice. In Aging cell, 23, e14314. doi:10.1111/acel.14314. https://pubmed.ncbi.nlm.nih.gov/39225086/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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