C57BL/6NCya-Dnah10em1/Cya
Common Name
Dnah10-KO
Product ID
S-KO-10710
Backgroud
C57BL/6NCya
Strain ID
KOCMP-56087-Dnah10-B6N-VA
Status
When using this mouse strain in a publication, please cite “Dnah10-KO Mouse (Catalog S-KO-10710) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Dnah10-KO
Strain ID
KOCMP-56087-Dnah10-B6N-VA
Gene Name
Product ID
S-KO-10710
Gene Alias
Dnahc10
Background
C57BL/6NCya
Gene Full Name
dynein, axonemal, heavy chain 10
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 5 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000058440
NCBI Transcript ID
NM_019536
Target Region
Exon 2~5
Size of Effective Region
~4.7 kb
Overview of Gene Research
DNAH10, short for dynein axonemal heavy chain 10, encodes a subunit of the inner arm dynein heavy chain in motile cilia and sperm flagella. It is crucial for ciliary and flagellar function, with its associated pathways likely involving those related to cilia-mediated processes. Genetic models, such as mouse models, are valuable for studying its functions [2].
In human and mouse models, DNAH10 deficiency has been shown to cause primary ciliary dyskinesia (PCD). A novel DNAH10 homozygous variant in a PCD patient led to sinusitis, bronchiectasis, situs inversus, and asthenoteratozoospermia. Immunostaining and electron microscopy revealed absence of related proteins and axoneme defects. Dnah10-knockin and-knockout mice recapitulated PCD phenotypes including chronic respiratory infection, male infertility, and hydrocephalus [2]. Also, bi-allelic DNAH10 variants in humans and mice cause asthenoteratozoospermia and male infertility due to sperm flagellar defects, specifically inner dynein arm deficiency [1,3].
In conclusion, DNAH10 is essential for the assembly and function of sperm flagella and motile cilia. The study of Dnah10-KO and-CKO mouse models has significantly contributed to understanding its role in diseases like PCD and male infertility, providing insights into the genetic basis and potential therapeutic strategies for these conditions.
References:
1. Li, Kuokuo, Wang, Guanxiong, Lv, Mingrong, He, Xiaojin, Cao, Yunxia. 2021. Bi-allelic variants in DNAH10 cause asthenoteratozoospermia and male infertility. In Journal of assisted reproduction and genetics, 39, 251-259. doi:10.1007/s10815-021-02306-x. https://pubmed.ncbi.nlm.nih.gov/34657236/
2. Wang, Rongchun, Yang, Danhui, Tu, Chaofeng, Qing, Jie, Luo, Hong. 2023. Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice. In Frontiers of medicine, 17, 957-971. doi:10.1007/s11684-023-0988-8. https://pubmed.ncbi.nlm.nih.gov/37314648/
3. Tu, Chaofeng, Cong, Jiangshan, Zhang, Qianjun, Cao, Yunxia, Tan, Yue-Qiu. 2021. Bi-allelic mutations of DNAH10 cause primary male infertility with asthenoteratozoospermia in humans and mice. In American journal of human genetics, 108, 1466-1477. doi:10.1016/j.ajhg.2021.06.010. https://pubmed.ncbi.nlm.nih.gov/34237282/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
