C57BL/6JCya-Rdh11em1/Cya
Common Name
Rdh11-KO
Product ID
S-KO-17671
Backgroud
C57BL/6JCya
Strain ID
KOCMP-17252-Rdh11-B6J-VB
Status
When using this mouse strain in a publication, please cite “Rdh11-KO Mouse (Catalog S-KO-17671) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Rdh11-KO
Strain ID
KOCMP-17252-Rdh11-B6J-VB
Gene Name
Product ID
S-KO-17671
Gene Alias
Mdt1, Psdr1, SCALD, ralR1, Arsdr1, CGI-82, HCBP12, M42C60, Ube-1c, UBE-1c1, 2610319N22Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 12 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000161204
NCBI Transcript ID
NM_021557
Target Region
Exon 2~3
Size of Effective Region
~2.5 kb
Overview of Gene Research
Rdh11, a retinol dehydrogenase, is an NADPH-dependent retinaldehyde reductase. It functions in the visual cycle, particularly in the oxidation of 11-cis-retinol to 11-cis-retinal in the retinal pigment epithelium (RPE), and also contributes to retinol homeostasis in extraocular tissues like the liver and testis [1,2,3]. It has dual specificity for both cis-and trans-retinoid substrates and may be involved in all-trans-and 9-cis-retinoic acid production [4].
In Rdh11 knockout mice, several phenotypes were observed. Rdh11 -/- embryos from Rdh11 -/- dams had elevated levels of embryonic retinyl esters [1]. Rdh11 -/- mice showed delayed dark adaptation, indicating its role in regenerating visual pigment by complementing RDH5 as an 11-cis-RDH in RPE cells [2]. In addition, microsomes from the testes and livers of Rdh11 -/- mice had lower rates of all-trans-retinaldehyde conversion to all-trans-retinol, and these tissues had lower levels of all-trans-retinol under a vitamin A-deficient diet, suggesting its essential role in maintaining retinol homeostasis in these tissues [3].
In conclusion, Rdh11 is crucial for visual processes, especially dark adaptation, and for maintaining retinol homeostasis in both ocular and extraocular tissues. The study of Rdh11 knockout mouse models has provided valuable insights into its functions, which may be relevant to understanding certain retinopathies and metabolic disorders related to vitamin A metabolism [1,2,3].
References：
1. Wu, Lizhi, Kedishvili, Natalia Y, Belyaeva, Olga V. 2019. Retinyl esters are elevated in progeny of retinol dehydrogenase 11 deficient dams. In Chemico-biological interactions, 302, 117-122. doi:10.1016/j.cbi.2019.01.041. https://pubmed.ncbi.nlm.nih.gov/30731079/
2. Kim, Tom S, Maeda, Akiko, Maeda, Tadao, Palczewski, Krzysztof, Nelson, Peter S. 2005. Delayed dark adaptation in 11-cis-retinol dehydrogenase-deficient mice: a role of RDH11 in visual processes in vivo. In The Journal of biological chemistry, 280, 8694-704. doi:. https://pubmed.ncbi.nlm.nih.gov/15634683/
3. Belyaeva, Olga V, Wu, Lizhi, Shmarakov, Igor, Nelson, Peter S, Kedishvili, Natalia Y. 2018. Retinol dehydrogenase 11 is essential for the maintenance of retinol homeostasis in liver and testis in mice. In The Journal of biological chemistry, 293, 6996-7007. doi:10.1074/jbc.RA117.001646. https://pubmed.ncbi.nlm.nih.gov/29567832/
4. Haeseleer, Françoise, Jang, Geeng-Fu, Imanishi, Yoshikazu, Nelson, Peter S, Palczewski, Krzysztof. 2002. Dual-substrate specificity short chain retinol dehydrogenases from the vertebrate retina. In The Journal of biological chemistry, 277, 45537-45546. doi:10.1074/jbc.M208882200. https://pubmed.ncbi.nlm.nih.gov/12226107/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
