C57BL/6JCya-Hdcem1/Cya
Common Name
Hdc-KO
Product ID
S-KO-18364
Backgroud
C57BL/6JCya
Strain ID
KOCMP-15186-Hdc-B6J-VB
Status
When using this mouse strain in a publication, please cite “Hdc-KO Mouse (Catalog S-KO-18364) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Hdc-KO
Strain ID
KOCMP-15186-Hdc-B6J-VB
Gene Name
Product ID
S-KO-18364
Gene Alias
Hdc-a, Hdc-c, Hdc-e, Hdc-s
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000028838
NCBI Transcript ID
NM_008230
Target Region
Exon 2
Size of Effective Region
~2.0 kb
Overview of Gene Research
Hdc, or histidine decarboxylase, is a rate-limiting enzyme for histamine synthesis. Histamine is a critical mediator of anaphylaxis, a neurotransmitter, and a regulator of gastric acid secretion. Hdc is expressed both in the posterior hypothalamus and peripherally, and its encoded enzyme is essential for histamine biosynthesis, which is involved in various biological processes such as immune regulation and neurotransmission [3,4]. Gene knockout mouse models have been valuable in studying Hdc's function.
In Hdc -/- mice, neutrophils are disorderly recruited into the ischemic injured area of the myocardium. Hdc deficiency leads to attenuated adhesion but enhanced migration and augmented ROS/NETs production in neutrophils, with Hdc -/- mouse-derived NETs promoting cardiomyocyte death and cardiac fibroblast proliferation/migration. Also, protein arginine methyltransferase 1 (PRMT1) is increased in Hdc -/- mouse-derived neutrophils [1].
In another study, Hdc-expressing myeloid-derived suppressor cells were found to promote basal-like transition and metastasis of breast cancer, and targeted ablation of Hdc+ PMN-MDSCs-derived Wnts hampered the metastatic cascade [2]. Chronic stress downregulates Hdc expression in ovarian cancer tumors, accelerating cancer growth, while exogenous histamine treatment alleviates this effect [5].
In Hdc knockout mice, genes associated with oligodendrocytes and myelin production are upregulated in the dorsal striatum, and there is reduced fractional anisotropy in the dorsal striatum, which may be related to the pathophysiology of Tourette syndrome [6]. Histamine deficiency in Hdc knockout mice exacerbates cisplatin-induced ferroptosis in cochlea hair cells [7].
In conclusion, Hdc is crucial for histamine synthesis and plays significant roles in multiple biological processes and disease conditions. The use of Hdc knockout mouse models has revealed its functions in cardiovascular diseases, breast cancer, ovarian cancer, Tourette syndrome-related pathophysiology, and cisplatin-induced ototoxicity, providing insights into disease mechanisms and potential therapeutic targets.
References：
1. Zhang, Zhiwei, Ding, Suling, Wang, Zhe, Yang, Xiangdong, Ge, Junbo. 2021. Prmt1 upregulated by Hdc deficiency aggravates acute myocardial infarction via NETosis. In Acta pharmaceutica Sinica. B, 12, 1840-1855. doi:10.1016/j.apsb.2021.10.016. https://pubmed.ncbi.nlm.nih.gov/35847488/
2. Chen, Na, Feng, Qiong, Deng, Jun, Hu, Jin-Ping, Deng, Huan. 2020. Hdc-expressing myeloid-derived suppressor cells promote basal-like transition and metastasis of breast cancer. In International journal of clinical and experimental pathology, 13, 1431-1443. doi:. https://pubmed.ncbi.nlm.nih.gov/32661481/
3. Li, Yapeng, Gao, Junfeng, Zhao, Dianzheng, Finkelman, Fred D, Huang, Hua. 2023. The Hdc GC box is critical for Hdc gene transcription and histamine-mediated anaphylaxis. In The Journal of allergy and clinical immunology, 152, 195-204.e3. doi:10.1016/j.jaci.2023.01.031. https://pubmed.ncbi.nlm.nih.gov/36804390/
4. Schneider, Erich H, Neumann, Detlef, Seifert, Roland. 2014. Modulation of behavior by the histaminergic system: lessons from HDC-, H3R- and H4R-deficient mice. In Neuroscience and biobehavioral reviews, 47, 101-21. doi:10.1016/j.neubiorev.2014.07.020. https://pubmed.ncbi.nlm.nih.gov/25102165/
5. Chen, Zhicong, Cao, Jinming, Xiao, Zhijun, Zhou, Ting, Xu, Feng. 2024. HDC downregulation induced by chronic stress promotes ovarian cancer progression via the IL-6/STAT3/S100A9 pathway. In Frontiers in pharmacology, 15, 1485885. doi:10.3389/fphar.2024.1485885. https://pubmed.ncbi.nlm.nih.gov/39720595/
6. Jindachomthong, Kantiya, Yang, Chengran, Huang, Yuegao, Frick, Luciana, Pittenger, Christopher. 2022. White matter abnormalities in the Hdc knockout mouse, a model of tic and OCD pathophysiology. In Frontiers in molecular neuroscience, 15, 1037481. doi:10.3389/fnmol.2022.1037481. https://pubmed.ncbi.nlm.nih.gov/36504678/
7. Wu, Daquan, Zhu, Baoling, Yang, Xiyang, Yang, Xiangdong, Huang, Xinsheng. 2024. Histamine deficiency exacerbates cisplatin-induced ferroptosis in cochlea hair cells of HDC knockout mice. In International immunopharmacology, 138, 112639. doi:10.1016/j.intimp.2024.112639. https://pubmed.ncbi.nlm.nih.gov/38972209/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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