C57BL/6JCya-Ddx23em1/Cya
Common Name
Ddx23-KO
Product ID
S-KO-18662
Backgroud
C57BL/6JCya
Strain ID
KOCMP-74351-Ddx23-B6J-VB
Status
When using this mouse strain in a publication, please cite “Ddx23-KO Mouse (Catalog S-KO-18662) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ddx23-KO
Strain ID
KOCMP-74351-Ddx23-B6J-VB
Gene Name
Product ID
S-KO-18662
Gene Alias
3110082M05Rik, 4921506D17Rik
Background
C57BL/6JCya
Gene Full Name
DEAD box helicase 23
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 15 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000003450
NCBI Transcript ID
NM_001080981
Target Region
Exon 4~13
Size of Effective Region
~5.2 kb
Overview of Gene Research
Ddx23, also known as PRP28, is a spliceosomal RNA helicase involved in pre-mRNA splicing [1]. It is part of the spliceosomal complex, and its ATPase activity is crucial for the pre-B to B spliceosome complex transition and for the release of U1 snRNP from the 5' splice site [1]. Besides RNA processing, mammalian Ddx23 is also implicated in antiviral responses, suggesting its importance in multiple biological pathways [2,3].
In fish, knockdown of black carp Ddx23 (bcDDX23) enhanced host cells' resistance against SVCV, indicating bcDDX23 negatively regulates MAVS-mediated antiviral signaling [2]. In human cells, knockdown of DDX23 enhanced VSV replication and reduced NF-κB and IRF3 activation, and DDX23 translocated to the cytoplasm to form complexes with TRIF or MAVS upon stimulation [3]. In ovarian cancer, silencing DDX23 impeded cell proliferation/invasion in vitro and tumor growth in vivo, as it regulated FOXM1 mRNA processing [4]. Also, in non-small-cell lung cancers, DDX23 can bind to the promoter of linc00630 to up-regulate its RNA level, forming an axis with linc00630-HDAC1 to promote metastasis [5].
In conclusion, Ddx23 is essential for pre-mRNA splicing and has significant roles in antiviral immunity and cancer progression. Functional studies, especially those using knockdown models, have revealed its importance in these biological processes and disease conditions, providing insights into potential therapeutic targets for viral diseases and certain cancers.
References：
1. Segovia, Danilo, Adams, Dexter W, Hoffman, Nickolas, Joshua-Tor, Leemor, Krainer, Adrian R. 2024. SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2322974121. doi:10.1073/pnas.2322974121. https://pubmed.ncbi.nlm.nih.gov/38743621/
2. Qin, Wei, Liu, Yankai, Xiao, Jun, Zhang, Yongan, Feng, Hao. 2023. DDX23 of black carp negatively regulates MAVS-mediated antiviral signaling in innate immune activation. In Developmental and comparative immunology, 146, 104727. doi:10.1016/j.dci.2023.104727. https://pubmed.ncbi.nlm.nih.gov/37164277/
3. Ruan, Jie, Cao, Yange, Ling, Tao, Xu, Anlong, Yuan, Shaochun. 2019. DDX23, an Evolutionary Conserved dsRNA Sensor, Participates in Innate Antiviral Responses by Pairing With TRIF or MAVS. In Frontiers in immunology, 10, 2202. doi:10.3389/fimmu.2019.02202. https://pubmed.ncbi.nlm.nih.gov/31620127/
4. Zhao, Chen, Li, Yingwei, Qiu, Chunping, Song, Kun, Kong, Beihua. 2021. Splicing Factor DDX23, Transcriptionally Activated by E2F1, Promotes Ovarian Cancer Progression by Regulating FOXM1. In Frontiers in oncology, 11, 749144. doi:10.3389/fonc.2021.749144. https://pubmed.ncbi.nlm.nih.gov/34966670/
5. Mao, Guozhang, Jin, Hui, Wu, Liuguang. . DDX23-Linc00630-HDAC1 axis activates the Notch pathway to promote metastasis. In Oncotarget, 8, 38937-38949. doi:10.18632/oncotarget.17156. https://pubmed.ncbi.nlm.nih.gov/28473661/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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