C57BL/6JCya-Pou2af1em1/Cya
Common Name
Pou2af1-KO
Product ID
S-KO-18701
Backgroud
C57BL/6JCya
Strain ID
KOCMP-18985-Pou2af1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Pou2af1-KO Mouse (Catalog S-KO-18701) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Pou2af1-KO
Strain ID
KOCMP-18985-Pou2af1-B6J-VA
Gene Name
Product ID
S-KO-18701
Gene Alias
Bob1, OCAB, BOB.1, Bob-1, OBF-1, OBF.1, OCA-B
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000034554
NCBI Transcript ID
NM_011136
Target Region
Exon 2~4
Size of Effective Region
~1.7 kb
Overview of Gene Research
POU2AF1, also known as OCA-B, is a transcription co-factor. It plays diverse roles in multiple biological processes. It is involved in pathways related to cell differentiation, immune response regulation, and adipogenesis. Its functions are crucial for normal physiological activities and its dysregulation may lead to various diseases [1,2,3,5,6,7].
In overweight patients, POU2AF1 expression is upregulated in retroperitoneal adipose tissue and promotes mesenchymal stem cells (MSCs) adipogenesis by inhibiting HDAC1 expression. Silencing endogenous POU2AF1 in MSCs inhibits adipogenesis [2]. In the context of Tfh cell maturation, Pou2af1 promotes Tfh formation and migration into the germinal center by upregulating CXCR5 [3]. In B-cell malignancies, B-cell malignancies dependent on POU2AF1 are sensitive to mSWI/SNF ATPase degraders, with treatment leading to chromatin eviction of POU2AF1 [4]. In CD4+ T cells, ectopic OCA-B (POU2AF1) expression improves antiviral memory recall responses and endogenously elevated OCA-B expression can prospectively identify memory precursor cells [5]. In the human airway epithelium, POU2AF1 helps maintain a host defense tone by regulating host-defense genes, and its expression is suppressed by cigarette smoke [6]. In chronic obstructive pulmonary disease (COPD), POU2AF1 may be a key regulator of emphysema, contributing to disease progression by regulating B-cell immunology [7].
In summary, POU2AF1 is a multifunctional transcription co-factor involved in adipogenesis, immune cell regulation, and host defense. Studies, including those using gene-knockout or conditional-knockout models in some cases, have revealed its role in diseases such as adiposity-related conditions, certain malignancies, and respiratory diseases, providing insights into potential therapeutic targets.
References：
1. Li, Xin, Zhou, Hongxu, Ma, Ruiyang, Zhong, Yifan, Jing, Zuoqian. 2024. Structure of POU2AF1 recombinant protein and it affects the progression and treatment of liver cancer based on WGCNA and molecular docking analysis. In International journal of biological macromolecules, 278, 134629. doi:10.1016/j.ijbiomac.2024.134629. https://pubmed.ncbi.nlm.nih.gov/39128756/
2. Wang, Yaqing, Wang, Luyang, Su, Zhongping, Jiang, Li, Kong, Xiangqing. . POU2AF1 promotes MSCs adipogenesis by inhibiting HDAC1 expression. In Adipocyte, 10, 251-263. doi:10.1080/21623945.2021.1918863. https://pubmed.ncbi.nlm.nih.gov/33949290/
3. Zhu, Xiaoliang, Chen, Xi, Cao, Yaqiang, Zhao, Keji, Zhu, Jinfang. 2024. Optimal CXCR5 Expression during Tfh Maturation Involves the Bhlhe40-Pou2af1 Axis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.05.16.594397. https://pubmed.ncbi.nlm.nih.gov/38903096/
4. He, Tongchen, Xiao, Lanbo, Qiao, Yuanyuan, Vakoc, Christopher R, Chinnaiyan, Arul M. 2024. Targeting the mSWI/SNF complex in POU2F-POU2AF transcription factor-driven malignancies. In Cancer cell, 42, 1336-1351.e9. doi:10.1016/j.ccell.2024.06.006. https://pubmed.ncbi.nlm.nih.gov/39029462/
5. Sun, Wenxiang, Hughes, Erik P, Kim, Heejoo, Williams, Matthew A, Tantin, Dean. 2024. OCA-B/Pou2af1 is sufficient to promote CD4+ T cell memory and prospectively identifies memory precursors. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2309153121. doi:10.1073/pnas.2309153121. https://pubmed.ncbi.nlm.nih.gov/38386711/
6. Zhou, Haixia, Brekman, Angelika, Zuo, Wu-Lin, Crystal, Ronald G, Wang, Guoqing. 2016. POU2AF1 Functions in the Human Airway Epithelium To Regulate Expression of Host Defense Genes. In Journal of immunology (Baltimore, Md. : 1950), 196, 3159-67. doi:10.4049/jimmunol.1502400. https://pubmed.ncbi.nlm.nih.gov/26927796/
7. Zhang, Da-Wei, Ye, Jing-Jing, Sun, Ying, Wei, Yuan-Yuan, Fei, Guang-He. 2022. CD19 and POU2AF1 are Potential Immune-Related Biomarkers Involved in the Emphysema of COPD: On Multiple Microarray Analysis. In Journal of inflammation research, 15, 2491-2507. doi:10.2147/JIR.S355764. https://pubmed.ncbi.nlm.nih.gov/35479834/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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