C57BL/6JCya-Il21em1/Cya
Common Name
Il21-KO
Product ID
S-KO-19049
Backgroud
C57BL/6JCya
Strain ID
KOCMP-60505-Il21-B6J-VA
Status
When using this mouse strain in a publication, please cite “Il21-KO Mouse (Catalog S-KO-19049) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Il21-KO
Strain ID
KOCMP-60505-Il21-B6J-VA
Gene Name
Product ID
S-KO-19049
Gene Alias
IL-21
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000029273
NCBI Transcript ID
NM_021782
Target Region
Exon 3~5
Size of Effective Region
~5.1 kb
Overview of Gene Research
Il21, or interleukin-21, is a cytokine that plays a crucial role in the immune system. CD4 T cells are the primary producers of Il21, often with the Il21-producing CD4 T cells expressing molecules associated with follicular helper cells (TFH). Il21 is involved in promoting effector functions and memory differentiation of CD8 T cells, and its signaling is associated with various autoimmune diseases [2].
In the expansion of human natural killer (NK) cells, feeder cells expressing membrane-bound (mb) Il21 alone drive superior yield compared to mbIL15, but mbIL15-based feeder cells drive superior functionality by most measures. However, sequential exposure to mbIl21 followed by mbIL15 is advantageous in achieving significant yields of highly functional NK cells [1].
In the context of autoimmune diseases, elevated Il21 is common in patients' tissues and sera, and CD4 T cell-derived Il21:Il21R signaling in CD8 T cells may have implications for several autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and others [2]. In shoulder arthritis, Il21 inhibits miR-361-5p, promoting MAP3K9 and aggravating the disease progression [3]. In chronic viral infection, Tfh-cell-derived Il21 sustains effector CD8+ T cell responses [4].
In conclusion, Il21 has diverse functions in the immune system, influencing NK cell expansion, T cell-mediated immune responses, and being associated with autoimmune diseases and arthritis. Studies on Il21 help to understand the immune-related biological processes and the mechanisms of associated diseases, potentially providing new strategies for treatment.
References：
1. Zhang, Caimei, Kadu, Siddhant, Xiao, Yansen, June, Carl H, Sheppard, Neil C. . Sequential Exposure to IL21 and IL15 During Human Natural Killer Cell Expansion Optimizes Yield and Function. In Cancer immunology research, 11, 1524-1537. doi:10.1158/2326-6066.CIR-23-0151. https://pubmed.ncbi.nlm.nih.gov/37649085/
2. Ren, Heather M, Lukacher, Aron E, Rahman, Ziaur S M, Olsen, Nancy J. 2021. New developments implicating IL-21 in autoimmune disease. In Journal of autoimmunity, 122, 102689. doi:10.1016/j.jaut.2021.102689. https://pubmed.ncbi.nlm.nih.gov/34224936/
3. Hao, Kangning, Lin, Pengchao, Li, Jing, Wang, Jiangyong, Li, Fei. 2024. IL21 inhibits miR-361-5p to promote MAP3K9 and further aggravate the progression of shoulder arthritis. In Aging, 16, 7915-7927. doi:10.18632/aging.205793. https://pubmed.ncbi.nlm.nih.gov/38728237/
4. Zander, Ryan, Kasmani, Moujtaba Y, Chen, Yao, Zajac, Allan, Cui, Weiguo. 2022. Tfh-cell-derived interleukin 21 sustains effector CD8+ T cell responses during chronic viral infection. In Immunity, 55, 475-493.e5. doi:10.1016/j.immuni.2022.01.018. https://pubmed.ncbi.nlm.nih.gov/35216666/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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