C57BL/6JCya-Aplp2em1/Cya
Common Name
Aplp2-KO
Product ID
S-KO-20514
Backgroud
C57BL/6JCya
Strain ID
KOCMP-11804-Aplp2-B6J-VB
Status
When using this mouse strain in a publication, please cite “Aplp2-KO Mouse (Catalog S-KO-20514) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Aplp2-KO
Strain ID
KOCMP-11804-Aplp2-B6J-VB
Gene Name
Product ID
S-KO-20514
Gene Alias
CDEBP, APLP-2
Background
C57BL/6JCya
Gene Full Name
amyloid beta (A4) precursor-like protein 2
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 9 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000072634
NCBI Transcript ID
NM_001102455
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Overview of Gene Research
Aplp2, also known as amyloid precursor-like protein 2, belongs to the APP family. It shares a similar structural organization with APP and APLP1, and has partially overlapping functions. APP family proteins are essential for survival. Aplp2 is involved in multiple biological processes and signaling pathways. For instance, it may play a role in TGF-β signaling [1], JNK-dependent cell migration [2], and is also associated with nociceptive information processing [3], mycobacterial infection defense [4], neuronal stem cell differentiation [5], refractive error and myopia development [6], and is related to the prognosis and clinicopathology of renal cell carcinoma [7], as well as lipid levels in the Chinese population in Xinjiang [8], and the regulation of MHC class I molecules on irradiated Ewing's sarcoma cells [9].
In mouse models, Aplp2 knockout mice develop high degrees of hyperopia and show a dose-dependent reduction in susceptibility to environmentally induced myopia, suggesting its important role in refractive development [6]. In addition, targeted knockdown of Aplp2 in GABAergic interneurons of GAD2-Cre mice evokes pain hypersensitivity by means of microglia activation, revealing its role in pain sensitivity regulation [3]. Mice with Aplp2 mutation or knockdown exhibit diminished macrophage-mediated killing of Mycobacterium tuberculosis, accompanied by decreased inducible nitric oxide synthase (iNOS) expression and reduced cytokine production, indicating its role in mycobacterial infection defense [4].
In conclusion, Aplp2 is crucial for multiple biological functions including cell migration, pain sensitivity, mycobacterial infection defense, and refractive development. The use of gene knockout or knockdown mouse models has significantly contributed to understanding its role in these biological processes and related disease conditions such as myopia, neuropathic pain, and tuberculosis.
References：
1. Tuersuntuoheti, Amannisa, Li, Qinshan, Teng, Yu, Wu, Wei, Song, Wei. 2023. YWK-II/APLP2 inhibits TGF-β signaling by interfering with the TGFBR2-Hsp90 interaction. In Biochimica et biophysica acta. Molecular cell research, 1870, 119548. doi:10.1016/j.bbamcr.2023.119548. https://pubmed.ncbi.nlm.nih.gov/37479189/
2. Wang, Xingjun, Guo, Xiaowei, Ma, Yeqing, Li, Wenzhe, Xue, Lei. 2018. APLP2 Modulates JNK-Dependent Cell Migration in Drosophila. In BioMed research international, 2018, 7469714. doi:10.1155/2018/7469714. https://pubmed.ncbi.nlm.nih.gov/30155482/
3. Li, Yu-Zhe, Zhu, Yue-Bin, Ge, An-Na, Bai, Hu-Hu, Wu, Shu-Jin. 2022. Reduced expression of APLP2 in spinal GABAergic inhibitory neurons contributed to nerve injury-induced microglial activation and pain sensitization. In Neuropharmacology, 224, 109334. doi:10.1016/j.neuropharm.2022.109334. https://pubmed.ncbi.nlm.nih.gov/36442651/
4. Chen, Jianxia, Tang, Fen, Li, Haohao, Wang, Peng, Liu, Feng. 2023. Mycobacterium tuberculosis suppresses APLP2 expression to enhance its survival in macrophage. In International immunopharmacology, 124, 111058. doi:10.1016/j.intimp.2023.111058. https://pubmed.ncbi.nlm.nih.gov/37844466/
5. Shariati, S Ali M, Lau, Pierre, Hassan, Bassem A, De Strooper, Bart, Gärtner, Annette. 2013. APLP2 regulates neuronal stem cell differentiation during cortical development. In Journal of cell science, 126, 1268-77. doi:10.1242/jcs.122440. https://pubmed.ncbi.nlm.nih.gov/23345401/
6. Tkatchenko, Andrei V, Tkatchenko, Tatiana V, Guggenheim, Jeremy A, Thinakaran, Gopal, Williams, Cathy. 2015. APLP2 Regulates Refractive Error and Myopia Development in Mice and Humans. In PLoS genetics, 11, e1005432. doi:10.1371/journal.pgen.1005432. https://pubmed.ncbi.nlm.nih.gov/26313004/
7. Gao, Lijian, Zhao, Hongda, Zhang, Dongqing, Xia, Yangyang, Shi, Benkang. 2018. Role of APLP2 in the prognosis and clinicopathology of renal cell carcinoma. In Oncology letters, 17, 508-513. doi:10.3892/ol.2018.9577. https://pubmed.ncbi.nlm.nih.gov/30655794/
8. Abuzhalihan, Jialin, Adi, Dilare, Wang, Yong-Tao, Fu, Zhen-Yan, Ma, Yi-Tong. . APLP2 gene polymorphisms are associated with high TC and LDL-C levels in Chinese population in Xinjiang, China. In Bioscience reports, 40, . doi:10.1042/BSR20200357. https://pubmed.ncbi.nlm.nih.gov/32716039/
9. Peters, Haley L, Yan, Ying, Solheim, Joyce C. 2013. APLP2 regulates the expression of MHC class I molecules on irradiated Ewing's sarcoma cells. In Oncoimmunology, 2, e26293. doi:. https://pubmed.ncbi.nlm.nih.gov/24353913/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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