| Model Generation Services | Species | 1 Project | ≥2 Projects | ≥3 Projects | Deliverables | |
|---|---|---|---|---|---|---|
| ES Cell Mediated | TurboKnockout | Mouse only | $3,000 off | $8,000 off | $14,000 off | ≥3 F1 animals |
| CRISPR/Cas9 - based | Knockout (KO) | Mouse or Rat | ≥3 F1 animals | |||
| KI (any locus) | ||||||
| KI (ROSA26) | ||||||
| Point mutation | ||||||
| cKO | ||||||
Spring into Summer Savings Event
Save up to $14k on select CAM projects
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Overview
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CAM Capabilities
Now is the perfect time to plan your next custom animal model (CAM) design project and lock-in tremendous savings for your research study!
The Spring into Summer Savings Event can help you extend your research by offering increased
discounts for multiple projects - save up to $14k on CRISPR- and embryonic stem (ES)
cell-mediated CAM for research. The more mouse or rat models you order, the more you save!
Plan Now - Start Later
Need more time? We can initiate projects based on your project timeline – so you can lock-in
promotional prices on multiple models and decide project delivery details later. Our Model
Design Specialists can provide complimentary project strategy details within 24 hours and
initiate your project as soon or as late as you require.
Spring into Summer Savings Event Details
This offer is applicable to all CRISPR- and embryonic stem (ES) cell-mediated custom animal model (CAM) projects. Transgenic animal model services are not eligible for this discount offer.
● Promotion Period: Offer ends June 30, 2022.
● Eligibility: Academic end clients in Europe, North America, and South America. Promotional pricing is only available for new projects with new clients.
Note
- ① Listed prices do not include handling fees or Taconic's RapidCheck Health Certification Program.
- ② Transgenic animal model projects do not qualify for this promotional offer.
- ③ This offer may not be combined with any other offer or discount.
Leverage Cyagen’s complete catalog of methodologies for generating novel mouse and rat models so that your research is not limited by available technologies. Whether your studies require the modification of an existing model or de novo generation of a complex or humanized model, we can match each project with the best methodology for success.
We can create any custom mouse or rat model for your project, from simple knockout (KO) and point mutation constructs, all the way through to more complicated constructs, such as conditional knockin (cKI) or humanization models.
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Knockouts (KO)
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Conditional Knockouts and Knock-ins (cKO/cKI)
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Point Mutation Knock-ins
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| Constitutive knockouts can be quickly generated utilizing TurboKnockout® embryonic stem cell (ESC) based technology providing ESC based models at timelines comparable to CRISPR based models. CRISPR is also available, with AI-based bioinformatic analysis driving identification of the optimal region to target. | Conditional knockouts and knock-ins can also be quickly generated using TurboKnockout®. With a germline guarantee and self-deleting selection cassette, TurboKnockout® accelerates traditional ESC based model generation. | Point mutations can be quickly generated via CRISPR or Cyagen’s accelerated ESC based method, TurboKnockout®, enabling more complex point mutations and/or genetic modifications. |
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Large-fragment Knock-ins (LFKI)
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Humanization
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Transgenics
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| Rosa26 and other safe harbor loci can be targeted for large fragment knock-ins (LFKI) using a variety of methodologies to ensure your studies are not limited by technology or capability. With the ability to perform LFKI at any locus, the only limit to your studies is your ingenuity. | Humanized models have been widely used in studies of human gene function, immune-oncology therapeutics, infectious disease pathology, and preclinical drug evaluation in the field of biomedicine. TurboKnockout® enables more efficient and stable gene integration for the development of humanized mouse models. | With the capability to generate both traditional and PiggyBac-based transgenics, we can provide your research with cost-effective transgenic models with the ability to avoid gene silencing with single- copy-per-integration-site, and no loss of transgene sequence when using PiggyBac. |
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Multiple Donor Strains Available ♦ Mouse: C57BL/6, FVB, BALB/c ♦ Rat: Sprague-Dawley (SD), Wistar, F344, Long evans, Brown Norway |
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TurboKnockout® - Proprietary Method for Accelerated Generation of Precise Mouse Models
Our proprietary TurboKnockout® gene targeting method can provide you with conditional knockout, reporter knockin, and humanization mouse models in as fast as 6-8 months. This proprietary gene targeting method is enabled by two innovations:
● Eliminates the unpredictability of germline transmission (GLT) with our super competent ESC line that generates 100% ESC-derived founder mice rather than chimeras.
● Self-removing selection cassette circumvents the need to breed to Flp deleter mice.
These innovations eliminate at least two generations of breeding, shortening production time by 4-6 months as compared to the industry standard.
Faster Turnaround with TurboKnockout®
TurboKnockout® gene targeting: 6-8 months
Highlights of TurboKnockout® Gene Targeting
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No patent risk:
Technology of choice for drug development projects
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Success rate:
100% guaranteed germline transmission (GLT)
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Turnaround time:
Founders as fast as 6-8 months
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Large fragment knockin size:
Knockin (KI) fragment size up to 200 kb
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Limitless loxP:
No size limitations for your floxed region
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100% Money-Back Guarantee on TurboKnockout® projects
Our Advantages
● Guaranteed Project Genotype
Cyagen’s gene editing services are unparalleled in delivering research-ready rodent models with a guaranteed genotype. To mitigate the risks associated with custom model generation, Cyagen provides a unique genotype guarantee* - confirmed using standard PCR validation of the expected fragment size to match the original project strategy - to give you further peace of mind.
*Does not guarantee biological outcome (i.e., transgene expression levels) and is limited by modifications that may negatively affect the health and viability.
● Comprehensive Capabilities
Provides researchers with access to a comprehensive toolkit of technologies, including but not limited to: CRISPR/Cas9, transgenic technology, PiggyBac, and embryonic stem cell (ESC)-based homologous recombination (HR).
● Fast Project Scoping & Quotes
We will work with you to identify the optimal CAM project strategy to serve your research goals and suit your budget. All CAM projects are OLAW-assured and AAALAC-accredited.
● Project Price Matching
Our team will price match custom animal model (CAM) project quotes from competitors and core facilities to help ensure researchers get the best deal for their study. With our Price Matching offer, we will provide a quote with the equivalent scope of work and an additional 5% discount. Bring your quote, we match it.
Contact us with your project details to request your complimentary model generation project consultation, strategy, and quote.
If you need immediate assistance, please email us at
animal-service@cyagen.com.
animal-service@cyagen.com.
