Cyagen’s CRISPR-based Rapid Knockout services use accelerated methodology that provides conventional knockout, reporter knockin, and humanization rat models in as fast as 5 months. This proprietary method is enabled by two innovations:
A proprietary AI-based bioinformatics analysis tool that identifies the optimal region
in the gene of interest while minimizing off-target effect.
● A proprietary pronuclear injection (PNI) cocktail and PNI technique that significantly enhances targeting efficiency.
These innovations allow the creation of knockout animals far more rapidly and reliably as compared to the industry standard. We typically produce CRISPR-mediated knockout rats in the Sprague-Dawley (SD) and Long Evans backgrounds, but we may be able to use other strains at your request.
- ● Knockout (KO)
- ● Point Mutation
- ● Large Fragment Knockin (LFKI)
- ● ROSA26 LFKI