As we review our accomplishments in 2020, we are delighted to share how our research partners have used our products and services to contribute to advances across numerous fields of study. In 2020 alone, Cyagen received at least 95 citations for our custom animal models - included among the 1,380 new research citations of our products and services. 

Herein, we have collected Cyagen’s top 10 research references from high impact factor (IF) journals to provide insights into a variety of mouse model types, gene targets, research applications, and some of the key developments shown in scientific literature throughout 2020. Additionally, we review complimentary resources offered by Cyagen to support researchers seeking to implement genetically engineered mouse or rat models, such as our AlphaKnockout Gene Targeting Tool and Letter of Support for grant funding applications.

 

Top Cyagen Research Citations of 2020

This ‘Top 10’ 2020 publication list features articles which use custom genetically modified mice developed by Cyagen. These custom mouse models are generated using a range of gene engineering techniques optimized for the unique project requirements. These citations cover a broad range of research fields, including immunology, inflammation, cardiology, oncology, metabolomics, and epigenetics – all with impact factor (IF) values greater than 20.

 

Top 10 Custom Mouse Model Publications from 2020

Mouse Model Type Used

Research Applications

Genetic/Molecular Target(s)

Title

Journal

Gene Knockout (KO)

Chronic Hepatitis B, Immunology

Signr1 (Cd209b)

Dual-targeting Nanoparticle Vaccine Elicits a Therapeutic Antibody Response Against Chronic Hepatitis B

Nature Nanotechnology

PMID: 32123380

Gene Knockout (KO)

Cardiology, cardiac hypertrophy

Cardiac-hypertrophy-associated piRNA (CHAPIR)

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N 6-methyladenosine methylation of Parp10 mRNA

Nature Cell Biology

PMID: 33020597

TurboKnockout® Gene Knockout (KO)

Aging biology, neurology, metabolism

Gm31629

Reducing Hypothalamic Stem Cell Senescence Protects against Aging-Associated Physiological Decline.

Cell Metabolism

PMID: 32004475

TurboKnockout® Humanized Point Mutation (PM)

Leukemia, oncology, tumors

Gata2 (G320D)

C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia Through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor

Cancer Cell

PMID: 32330454

Gene Knockin (KI)

Immunology

Irak2

IL-1 induces mitochondrial translocation of IRAK2 to suppress oxidative metabolism in adipocytes

Nature Immunology

PMID: 32778760

Gene Knockin (KI)

(CRISPR-mediated)

Metabolism, cholesterol

USP20

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis

Nature

PMID: 33177714

Gene Knockin (KI)

(CRISPR-mediated)

Epigenetics, metabolomics, oncology

Ezh2K735R

SETD2 Restricts Prostate Cancer Metastasis by Integrating EZH2 and AMPK Signaling Pathways

Cancer Cell

PMID: 32619406

PNI-based Transgenic

Stem cells, coronary disease, internal ribosomal entry site (IRES)

Myh11-DreERT2

Lgals3-IRES-Cre

The Stem Cell Pluripotency Genes Klf4 and Oct4 Regulate Complex SMC Phenotypic Changes Critical in Late-Stage Atherosclerotic Lesion Pathogenesis

Circulation

PMID: 32674599

Conditional Knockout (cKO) a.k.a. floxed gene

Immunology, Inflammation

 

NMBR

Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition

Nature Immunology

PMID: 32807943

Conditional Knockout (cKO) a.k.a. floxed gene

Macrophages, metabolism,

Slc16a1 (mct1)

Endothelial Lactate Controls Muscle Regeneration From Ischemia by Inducing M2-like Macrophage Polarization

Cell Metabolism

PMID: 32492393

 

Search Cyagen’s Citation List – Animal Models, Molecular & Cell Biology

In under 15 years since its founding, Cyagen has become a leading provider of custom mouse and rat models – delivering over 78,400 models to researchers worldwide and receiving over 4,750 peer-reviewed citations of our expansive range of products and services, which include:

  • Custom Rodent Models: TurboKnockout® gene targeting mice, Rosa26 Transgenic, Knockout (KO), floxed/cKO, Large-fragment Knockin (LFKI), point mutation, and much more
  • Cell line generation: knockout (KO), knockin, point mutation, overexpression
  • Cell products: research-use stem cells, media, cell culture supplements and reagents
  • DNA vector construction and BAC modification services
  • Virus packaging services

Genetically engineered models remain essential for gaining proper understanding of human disease mechanisms and play an important role in a variety of research areas. Thus, we created a comprehensive searchable list that includes all 4,750+ citations of Cyagen’s animal models, molecular biology, and cellular biology deliverables. New search features include animal model citation filters for species, project type, application, and more – see it in action: Search the Updated Citations Resource

 

Complimentary Research Support Available from Cyagen

Cyagen has developed many innovative services to provide unmatched economy, process ease, and efficiency - allowing scientists to get back to discovery. Our clients receive free consultation and strategy design from a team of PhD scientists that boast several decades of collective experience in the field. If you are considering the use of a genetically engineered mouse or rat model, we have developed the following complimentary services to support your research planning process.

AlphaKnockout - FREE Smart Gene Targeting System

Cyagen’s Artificial Intelligence (AI)-based gene targeting system designs highly efficient custom CRISPR and embryonic stem (ES) cell-mediated KO/cKO strategies in under 30 seconds. It is fast, intelligent, reliable, reproducible, and best of all, FREE! 

Query for your desired species (mouse/rat), gene targeting strategy (ES cell/CRISPR), and gene of interest to instantly receive project strategy designs. These rapidly generated AI-based reports provide targeting strategies, tandem repeat analysis, suitability for sequencing, and additional phenotypic data for consideration in your project plan. Try it today: www.alphaknockout.com/

Cyagen’s Letter of Support for Research Grant Proposals

Letters of support (LOS) can express information that may be a deciding factor in awarding funding when grant proposals are otherwise similar in merit and quality. Additionally, the inclusion of LOS shows that the investigator has prepared a comprehensive proposal and is prepared to begin the project when it is awarded.

Including a letter of support (LOS) from Cyagen quickly establishes the feasibility of the animal model project, providing a competitive advantage for your grant proposal. Contact us for your complimentary letter of support!

 

About Cyagen

Cyagen provides researchers from around the world with transgenicknockoutknockinconditional knockout models and also offers a comprehensive series of stem cell products for research use, including cell lines, media, and differentiation kits. Cyagen’s proprietary embryonic stem (ES) cell-based TurboKnockout® Gene Targeting is free of patent disputes, providing precise mouse models on a comparable timeline to CRISPR/Cas9-based techniques.

TurboKnockout® Gene Targeting Services

TurboKnockout® services can deliver conditional knockout (cKO/floxed), conventional knockout (KO), ROSA26 knockin, point mutation knockin (KI), reporter knockin, and humanization mouse models in as fast as 6 months. For TurboKnockout®-mediated conditional knockout (cKO/floxed) mice, it may be possible to breed founders directly to your tissue-specific Cre mice.

Advantages of TurboKnockout® Mouse Model Generation

  • Freedom to Operate: Technology of choice for drug development projects
  • Success Rate: 100% guaranteed germline transmission (GLT)
  • Turnaround Time: Founders as fast as 6-8 months
  • Large Fragment Knockin: Insert fragments up to 300 kb
  • Flexible Floxing: No size limitations for your floxed region
  • 100% Money-Back Guarantee 

Contact Us for Custom Model Generation Support

From vector and strategy design to animal model creation, cryopreservation, and breeding, Cyagen offers complete outsourcing for all animal model needs. In addition to our efficient and economical suite of technologies and services, we take pride in the ease of process we provide to our clients:

  • Streamlined process provides customers with project strategy & quotes within 48 hours of request
  • Reduced project timeline compared with most university core facilities
  • Transparency via robust reporting enables customers to drive strategy

 

To support all scientists – whether operating on a strict budget or working with picky procurement offices - our price matching policy guarantees you get the best deal. See how we can help your research: contact us with your gene target(s) and our custom model generation experts will be in touch with you.