Mouse Status: Homozygous knockout mice are developmentally normal and fertile
In this mouse homozygote, the J-segment of the Ig heavy chain locus is completely deleted, resulting in the inability of the cell to produce a recombinant version of the complete heavy chain variable region. The B cells of Ighj knockout (KO) mice have undergone dramatic changes in developmental process and cell number, which can be used as an animal model of B cell immune deficiency. Ighj KO mice retain other immune cells except B cells, so changes in other immune cells can be detected in Ighj KO mice.
• Detection of B cells
Figure 1. Detection of B cells in peripheral blood and spleen of Ighj KO homozygous mice
The results showed that B cell deletion was detected in the peripheral blood and spleen of Ighj KO homozygous mice.