Hexb-T2A-CreERT2 Mice

Catalog Number: C001580

Strain Name: C57BL/6JCya-Hexbem1(T2A-CreERT2)/Cya

Genetic Background: C57BL/6JCya

Examples of Expressing Tissues/Cells: Nervous System, Microglia

 

Strain Description

The Hexb-T2A-CreERT2 mouse was constructed by integrating the T2A-CreERT2 gene expression cassette into the mouse Hexb gene using gene-editing technology, allowing for the expression of CreERT2 recombinase under the control of the mouse Hexb gene regulatory elements. In the absence of tamoxifen treatment, CreERT2 recombinase is primarily located in the cytoplasm. Only under the action of tamoxifen can CreERT2 recombinase enter the nucleus and exert its recombination function. When Hexb-T2A-CreERT2 mice are crossed with mice containing loxP sites, tamoxifen induction is expected to trigger Cre recombinase-mediated sequence recombination between loxP sites in the nervous system (microglia) of the offspring.

Strain Strategy

The TAG stop codon will be replaced with the T2A-CreERT2 cassette. A synonymous mutation p. L525= (CTC to TTA) will also be introduced to prevent the binding and re-cutting of the sequence after homology-directed repair.



Announcement

The insertion site of the Cre recombinase gene expression cassette in this strain is located on chromosome 13. Please avoid breeding with gene-edited mice targeting genes on the same chromosome as the Cre mouse when conducting mating.