hTFRC/Ttn-KO Mouse
Product Name
hTFRC/Ttn-KO Mouse
Product ID
C001976
Strain Name
C57BL/6Cya-Ttnem1Tfrctm1(hTFRC)/Cya
Backgroud
C57BL/6Cya
Status
When using this mouse strain in a publication, please cite “hTFRC/Ttn-KO Mouse (Catalog C001976) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Alias
L56, mdm, shru, 1100001C23Rik, 2310036G12Rik, 2310057K23Rik, 2310074I15Rik, D330041I19Rik, D830007G01Rik, T9, TR, TFR, p90, CD71, TFR1, TRFR, IMD46
Chromosome
Chr 2, Chr 3
Datasheet
Strain Description
hTFRC/Ttn-KO mouse model is a model obtained by breeding Ttn-KO mice (Catalog No.: S-KO-05587) with hTFRC mice (Catalog No.: C001584). This model can be utilized in research for hereditary muscle diseases such as familial dilated cardiomyopathy (DCM), early-onset myopathy, and muscular dystrophy, supporting the development of TFRC-targeted gene supplementation therapies. Homozygous knockout of the Ttn gene is lethal, whereas heterozygous Ttn-KO mice are viable and fertile.
Strain Strategy
The hTFRC/Ttn-KO mice are a model obtained by mating Ttn-KO mice (catalog number: S-KO-05587) with hTFRC mice (catalog number: C001584).
Figure 1a. Diagram of the gene editing strategy for the generation of hTFRC mice.
*TFRC chimeric cDNA: Mouse cytoplasmic and helical-Human extracellular.
Figure 1b. Diagram of the gene editing strategy for the generation of hTFRC mice. TurboKnockout targeting technology was used to replace part of exon 2 of the mouse Tfrc gene with a human TFRC chimeric cDNA WPRE-BGH pA cassette. Gene-editing techniques were employed to knock out exons 10-13 of the mouse Tfrc gene.
Figure 2. Diagram of the gene editing strategy for the generation of Ttn-KO mice. Gene-editing techniques were employed to knock out exons 4-6 of the mouse Ttn gene.
Application Area
Screening, development, and evaluation of TFRC-targeted gene supplementation therapies;
Research on hereditary muscle diseases such as familial dilated cardiomyopathy (DCM), early-onset myopathy, and muscular dystrophy;
Research and evaluation of muscle drug delivery.
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.