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huHSC-NKG-hIL15 Mouse
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huHSC-NKG-hIL15 Mouse
Product Name
huHSC-NKG-hIL15 Mouse
Product ID
C001526
Strain Name
huCD34+HSC-NOD.Cg-PrkdcscidIl2rgem1Il15em1(hIL15)/Cya
Backgroud
NKG
Status
When using this mouse strain in a publication, please cite “huHSC-NKG-hIL15 Mouse (Catalog C001526) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
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Basic Information
Validation Data
Related Resource
Basic Information
Gene Name
IL15 & Il2rg
Gene Alias
IL-15, gc, p64, [g]c, CD132, gamma(c)
NCBI ID
Chromosome
Chr 4 (Human), Chr X (Mouse)
MGI ID
Datasheet
Strain Description
NKG mice (Catalog Number: C001316) are a type of severe immunodeficient mouse developed by Cyagen by deleting the Il2rg gene from the NOD-Scid strain. This strain lacks mature T, B, and NK cells, has reduced complement activity, and weak macrophage phagocytosis of human cells. As a result, NKG mice can efficiently engraft human hematopoietic stem cells (HSC), peripheral blood mononuclear cells (PBMC), patient-derived xenografts (PDX), or adult stem cells and tissues.
Interleukin-15 (IL-15) is a cytokine that regulates the activation and proliferation of T cells and natural killer (NK) cells. It also plays a role in balancing the number of CD8+ memory cells alongside IL-2. Research indicates that IL-15 is essential for the differentiation, function, and survival of NK cells. Providing sufficient human IL-15 can help stabilize the function of human NK cells within a mouse model [1-2]. The NKG-hIL15 mice (Catalog Number: C001513) are constructed by knocking in the human IL15 gene from the NKG mice. Compared to NKG mice, NKG-hIL15 mice significantly enhance the reconstitution proportion of human NK cells after HSC or PBMC transplantation. These mice can be utilized for the development of immunotherapies targeting NK cells and drug evaluation.
The huHSC-NKG-hIL15 mouse refers to an immune system humanized mouse model constructed by transplanting human hematopoietic stem cells (HSC) into NKG-hIL15 mice after sub-lethal dose irradiation. This model can reconstitute various immune cells, has a long lifespan, and is particularly effective in rebuilding human NK cells, which can aid in the development of NK cell-related tumor immunotherapies.
Reference
Katano I, Nishime C, Ito R, Kamisako T, Mizusawa T, Ka Y, Ogura T, Suemizu H, Kawakami Y, Ito M, Takahashi T. Long-term maintenance of peripheral blood derived human NK cells in a novel human IL-15- transgenic NOG mouse. Sci Rep. 2017 Dec 8;7(1):17230.
Huntington ND, Legrand N, Alves NL, Jaron B, Weijer K, Plet A, Corcuff E, Mortier E, Jacques Y, Spits H, Di Santo JP. IL-15 trans-presentation promotes human NK cell development and differentiation in vivo. J Exp Med. 2009 Jan 16;206(1):25-34.
Strain Strategy
Typically, 4 to 5-week-old female NKG-hIL15 mice are selected and after irradiation, they receive a tail vein injection of human CD34+ hematopoietic stem cells (HSC).
Application Area
Research on the human immune system, haematopoietic system;
Human-derived cell line xenograft (CDX) and patient-derived xenograft (PDX);
NK cell development mechanism studies, NK cell-related tumor immunotherapy development, and antibody-dependent NK cell-mediated toxicity (ADCC) studies.
Validation Data
Related Resource
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